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【目的】以前期研究小麦生境分离细菌为菌株库,从中筛选耐低温菌株,评价其植物促生效果。【方法】通过4°C低温培养筛选耐低温菌株。以产吲哚乙酸、1-氨基环丙烷羧酸(ACC)脱氨酶、嗜铁素以及无机、有机磷溶解能力为评价指标,测定15°C和28°C环境中耐低温菌株的植物促生作用潜力,利用赋分评估系统选定潜力菌株进行温室和田间试验验证其植物促生作用。【结果】筛选到34株耐低温菌株,从中选定的8株植物促生潜力细菌温室试验促生效果和评估分值之间的相关性在0.62以上,菌株1b YB22和3b JN2在28°C环境中对黄瓜的促生效果均在28%以上,二者在15°C环境中对青菜种子根长的促生效果分别为116.76%和46.82%,菌株1b YB22在15°C环境中对青菜的促生效果为25.11%。菌株1b YB22在田间应用对小麦生长同样具有促生作用,增产效果达0.58%。【结论】研究建立的筛选体系从小麦生境分离菌株中获得了耐低温荧光假单胞菌株1b YB22和3b JN2,两株细菌在15°C和28°C均具有植物促生作用,同时可以在田间促进小麦生长。
【Objective】 The objective of this study was to isolate the bacteria from the wheat habitat as a strain pool and screen the low temperature resistant strains to evaluate the plant growth promoting effect. 【Method】 Low temperature resistant strains were screened by low temperature culture at 4 ° C. To indoleacetic acid, 1 - aminocyclopropanecarboxylic acid (ACC) deaminase, and iron, and inorganic and organic phosphorus solubilization as an evaluation index, determination of 15 ° C and 28 ° C low temperature resistant plant strains Potential role of potential, the use of evaluation system points potential evaluation system for greenhouse and field experiments to verify the plant growth promoting role. 【Result】 The results showed that 34 strains of low temperature resistant strains were screened. Among them, 8 strains of plant growth promoting potential selected from them were higher than 0.62 for the test of promoting growth and the score of assessment, and those of strains 1b, YB22 and 3b JN2, The curative effect of cucumber in the environment was above 28%, both of them had 116.76% and 46.82% promoting effects on root length of cabbage in 15 ℃ environment. The promoting effect is 25.11%. The strain 1b YB22 also had the promoting effect on the growth of wheat in the field, and the yield was up to 0.58%. 【Conclusion】 The screening system established in this study obtained low temperature-resistant P. fluorescens strains 1b YB22 and 3b JN2 from isolates of wheat habitats. Both strains had plant growth promoting effects both at 15 ° C and 28 ° C, Field to promote wheat growth.