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目的:探讨木鳖子单体化合物对羟基桂皮醛(p-hydroxylcinnamaldehyde,CMSP)对食管癌细胞增殖及在BALB/c裸鼠移植瘤生长的抑制作用。方法:细胞计数法检测CMSP对食管癌细胞株Kyse 30和TE-13增殖的抑制作用,RT-PCR法检测食管癌细胞中CEA和SCC mRNA的表达情况,Western blotting法检测食管癌细胞中C-myc和N-myc蛋白的表达变化。构建裸鼠食管癌细胞移植瘤模型,分为对照组和CMSP处理组,实验结束后测量移植瘤大小及体积;免疫组织化学SP法观察移植瘤组织C-myc和N-myc蛋白的表达变化,H-E染色后光镜下观察两组裸鼠移植瘤组织和肝、脾组织的病理学变化。结果:CMSP对食管癌细胞Kyse 30和TE-13的增殖具有明显的抑制作用[(1.7±0.3)vs(3.8±0.3),(1.6±0.2)vs(4.5±0.4),均P<0.01]。经CMSP处理后:(1)Kyse 30和TE-13细胞中CEA和SCC mRNA表达降低(P<0.01)、C-myc和N-myc蛋白表达明显低于对照组(P<0.05或P<0.01);(2)裸鼠移植瘤平均体积和质量明显降低(P<0.05或P<0.01),移植瘤组织中Cmyc和N-myc蛋白表达水平低于盐水对照组肿瘤组织。CMSP处理组和对照组中肝、脾组织并未发生任何病理改变。结论:木鳖子单体化合物CMSP体外抑制食管癌细胞增殖,体内抑制裸鼠食管癌移植瘤的生长。
OBJECTIVE: To investigate the inhibitory effect of p-hydroxylcinnamaldehyde (CMSP) on the proliferation of esophageal cancer cells and the growth of xenografted BALB / c nude mice. Methods: The cytotoxicity of CMSP on the proliferation of esophageal carcinoma cell lines Kyse 30 and TE-13 was detected by cell counting method. The expression of CEA and SCC mRNA in esophageal cancer cells was detected by RT-PCR. The expression of C- myc and N-myc protein expression changes. The nude mice model of esophageal cancer xenografts was established and divided into control group and CMSP treatment group. The size and volume of the transplanted tumor were measured after the experiment. The expression of C-myc and N-myc in the xenografted tumor was observed by immunohistochemical SP method, The pathological changes of the xenografts in the nude mice and the liver and spleen tissues were observed under light microscope after HE staining. Results: CMSP could significantly inhibit the proliferation of esophageal cancer cells Kyse 30 and TE-13 [(1.7 ± 0.3) vs (3.8 ± 0.3) vs (1.6 ± 0.2) vs (4.5 ± 0.4), all P <0.01] . After CMSP treatment: (1) The expressions of CEA and SCC mRNA in Kyse 30 and TE-13 cells were decreased (P <0.01), and the expressions of C-myc and N-myc were significantly lower than those in control group (P <0.05 or P <0.01 ). (2) The average volume and mass of transplanted tumor in nude mice were significantly decreased (P <0.05 or P <0.01). The expression of Cmyc and N-myc in tumor tissue was lower than that in saline control group. CMSP treatment group and control group liver and spleen did not occur any pathological changes. Conclusion: CMML can inhibit the proliferation of esophageal cancer cells in vitro and inhibit the growth of esophageal carcinoma in nude mice in vivo.