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目的观察加巴喷丁(GBP)对吸入七氟烷(SEV)的小鼠的镇痛、催眠和遗忘作用的影响。方法采用热板法和甩尾法观察GBP对SEV小鼠痛阈(HPPT)的影响,翻正反射实验观察各实验组小鼠睡眠潜伏期、睡眠维持时间和入睡率,避暗和跳台实验观察潜伏期和错误次数。采用翻正反射法时按分层随机分组,分为GBP(G)组、SEV(S)组和GBP+SEV(GS)组,每组10只;采用甩尾法、热板法、避暗和跳台实验时按分层随机分组,分为0.9%氯化钠溶液(NS)组、G组、S组、GS组,每组10只。结果热板法实验中,GS组给药后15、25 min的HPPT值显著高于NS组、G组及S组(P值均<0.05);甩尾法实验中,GS组给药后10、15 min的HPPT值显著高于G组,5、10、15 min的HPPT值显著高于S组(P值均<0.01)。翻正反射法实验中,GS组的睡眠潜伏期较G组显著缩短(P<0.01),睡眠时间较G组及S组显著延长(P值分别<0.05、0.01),入睡率显著高于G组及S组(P值均<0.01)。跳台实验中,GS组的跳台潜伏期较G组显著缩短(P<0.05),错误次数较G组显著增多(P<0.01);避暗实验中,GS组的避暗潜伏期较S组显著缩短(P<0.05),错误次数较S组显著增多(P<0.05)。结论 GBP与SEV合用可增强小鼠的镇痛、催眠和遗忘作用。
Objective To observe the effects of gabapentin (GBP) on the analgesic, hypnotic and amnesic effects of inhaled sevoflurane (SEV) mice. Methods The effects of GBP on pain threshold (HPPT) in SEV mice were observed by hot plate method and tail-flick method. The sleep latency, sleep maintenance time and sleep rate of mice in each experimental group were observed by inverted-reflex test. And the number of errors. The patients were divided into GBP (G) group, SEV (S) group and GBP + SEV (GS) group with 10 cases in each group according to the stratified reflex method. The tail flick method, hot plate method, And jumping test were randomly divided into stratified by 0.9% sodium chloride solution (NS) group, G group, S group, GS group, 10 rats in each group. Results In hot plate test, the HPPT values at 15 and 25 min after GS administration were significantly higher than those in NS, G and S groups (all P <0.05). In the tail flick test, HPPT values at 15 min were significantly higher than those at G and HPPT values at 5, 10 and 15 min were significantly higher than those in S group (all P <0.01). In the normal-reflex test, the sleep latency of GS group was significantly shorter than that of G group (P <0.01), sleep time was significantly longer than that of G group and S group (P <0.05, 0.01 respectively), and the rate of falling asleep was significantly higher than that of G group And S group (P <0.01). The jumping latency of GS group was significantly shorter than that of G group (P <0.05), and the number of errors was significantly higher than that of G group (P <0.01). In darkness experiment, the latency of darkening of GS group was significantly shorter than that of S group P <0.05), the number of errors was significantly higher than that of S group (P <0.05). Conclusions GBP combined with SEV can enhance the analgesic, hypnotic and amnesic effects of mice.