[Objective] To investigate the effect of different methods on the extraction of rutin and quercetin in Rosa Laevigata Michx., so as to provide scientific basis for its reasonable development. [Method] Rutin and quercetin in Rosa Laevigata Michx. were extracted by hot water soaking, alcohol reflux and alcohol-ultrasonic assisted methods, and the contents of which were determined by HPLC, methodology investigation was carried out as well. [Result] Rutin and quercetin were separated well by HPLC, which had good relationships with peak areas in the range of 0.03-15 μg/ml (r=0.999 0) and 0.04-20 μg/ml(r=0.999 4) respectively, and the limits of detection (LOD) were 12.6 and 15.2 ng/ml. The extraction effect of the above three methods was in the order of alcohol-ultrasonic assisted method>alcohol reflux>hot water soaking, and the yield rates of rutin were 0.62, 1.04, 1.52 μg/ml respectively; the yield rates of quercetin were 0.78, 1.18 and 2.24 μg/ml.[Conclusion] Alcohol-ultrasonic assisted method was proved to be the best way to extract rutin and quercetin from Rosa Laevigata Michx. [Objective] To investigate the effect of different methods on the extraction of rutin and quercetin in Rosa Laevigata Michx., So as to provide scientific basis for its reasonable development. [Method] Rutin and quercetin in Rosa Laevigata Michx. soaking, alcohol reflux and alcohol-ultrasonic assisted methods, and the contents of which were determined by HPLC, methodology investigation was carried out as well. [Result] Rutin and quercetin were separated well by HPLC, which had good relationships with peak areas in the range of 0.03-15 μg / ml (r = 0.999 0) and 0.04-20 μg / ml (r = 0.999 4) respectively, and the limits of detection (LOD) were 12.6 and 15.2 ng / ml. above three methods was in the order of alcohol-ultrasonic assisted method> alcohol reflux> hot water soaking, and the yield rates of rutin were 0.62, 1.04, 1.52 μg / ml respectively; the yield rates of quercetin were 0.78, 1.18 and 2.24 μg /ml.[Conclusion] Alcohol-ultrasonic assist ed method was proved to be the best way to extract rutin and quercetin from Rosa Laevigata Michx.