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目的观察缺氧缺血性脑病(HIE)新生儿血清单核细胞趋化蛋白-1(MCP-1)和S100B蛋白的水平变化,探讨其在HIE中的临床意义。方法采用双抗体夹心酶联免疫吸附法检测60例足月HIE患儿(HIE组)及31例健康足月新生儿(健康对照组)出生24 h内血清MCP-1和S100B蛋白水平,比较各组间的差异;并分析HIE各组MCP-1和S100B蛋白的相关性。结果 1.HIE组血清MCP-1、S100B蛋白水平分别为(61.25±15.21)ng·L-1、(4.49±1.20)μg·L-1,明显高于健康对照组[(33.29±14.02)ng·L-1、(1.03±0.35)μg·L-1](t=11.88、12.59,Pa<0.01);2.轻度、中度与重度HIE组血清MCP-1水平分别为(45.23±14.53)ng·L-1、(60.62±17.23)ng·L-1、(74.25±14.01)ng·L-1,与健康对照组比较差异有统计学意义(F=89.07,P<0.01);轻度、中度与重度HIE组血清S100B蛋白水平分别为(2.42±0.60)μg·L-1、(4.62±1.05)μg·L-1、(6.54±0.86)μg·L-1,与健康对照组比较差异有统计学意义(F=91.11,P<0.01);3.HIE各组血清MCP-1和S100B蛋白水平均呈正相关(r=0.819、0.826、0.816,P<0.05)。结论血清MCP-1和S100B蛋白水平可作为判断HIE新生儿病情严重程度的早期指标之一。
Objective To investigate the changes of serum monocyte chemoattractant protein-1 (MCP-1) and S100B protein in neonates with hypoxic-ischemic encephalopathy (HIE) and to explore its clinical significance in HIE. Methods Serum levels of MCP-1 and S100B in 60 HIE infants and 31 healthy full-term newborns (healthy control group) were measured by double antibody sandwich enzyme-linked immunosorbent assay The differences between the two groups were analyzed. The correlations between MCP-1 and S100B proteins in HIE groups were analyzed. The serum levels of MCP-1 and S100B in HIE group were (61.25 ± 15.21) ng · L-1 and (4.49 ± 1.20) μg · L-1, respectively, which were significantly higher than those in healthy control group [(33.29 ± 14.02) ng (1.03 ± 0.35) μg · L-1] (t = 11.88,12.59, Pa <0.01). 2. The levels of serum MCP-1 in mild, moderate and severe HIE group were (45.23 ± 14.53 (60.62 ± 17.23) ng · L-1 and (74.25 ± 14.01) ng · L-1, respectively, which were significantly lower than those in healthy controls (F = 89.07, P <0.01) The serum levels of S100B protein in HIE group were (2.42 ± 0.60) μg · L-1, (4.62 ± 1.05) μg · L-1, (6.54 ± 0.86) μg · L-1, respectively. (F = 91.11, P <0.01). 3. The levels of serum MCP-1 and S100B protein in HIE groups were positively correlated (r = 0.819,0.826,0.816, P <0.05). Conclusion Serum MCP-1 and S100B protein levels may be used as one of the early indicators to judge the severity of neonatal HIE.