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为完善昆虫病原索科线虫线粒体基因组全序列数据库,更系统地研究其基因组特征和系统演化规律,进而为发挥该线虫生防潜力打下基础,我们开展了中华卵索线虫Ovomermis sinensis线粒体全基因组的研究。该研究通过线粒体基因组滚环复制及酶切图谱,揭示了中华卵索线虫线粒体基因组具有种内遗传多态性,即群体中单体线虫具有独特的酶切条带,且条带累加之和变化范围较大,为16.5～24.5kb。为进一步了解线粒体基因组多态性特征及产生的分子机制,采用两步长PCR方法对2条代表性成虫线粒体基因组进行了测序及拼接,得其全长分别为18864和16777bp。对这2条序列的比对表明,线粒体基因组中位于ND2和ND4之间的可变区域,不仅基因排列顺序不同,且存在ND3基因重复现象,这是导致中华卵索线虫线粒体基因组呈现多态性的主要原因。通过对以上研究结果的分析及与GenBank中已有的6种索科线虫线粒体基因组序列进行比对,概括出其线粒体基因组基本特点:①线粒体基因排列顺序各不相同;②部分线虫线粒体基因存在重复现象,且重复次数不同;③线粒体基因组大小存在很大差异。
In order to improve the genome sequence of the mitochondrial genome of the Entomopathogenic nematode and to systematically investigate its genomic characteristics and systematic evolutionary rules, and to lay the foundation for the potential of its biocontrol potential, we conducted a genome-wide mitochondrial genome study of Ovomermis sinensis . In this study, mitochondrial genome rolling circle replication and restriction map revealed that the Chinese Eggworm mitochondrial genome has an intraspecific genetic polymorphism, that group of monomeric nematodes with unique restriction endonuclease band, and the sum of the sum of the band changes A larger range of 16.5 ~ 24.5kb. In order to further understand the characteristics of mitochondrial genome polymorphism and its molecular mechanism, the two representative adult mitochondrial genomes were sequenced and spliced by using two-step PCR. The full-length cDNA sequences were 18864 and 16777 bp in length. The alignment of the two sequences showed that the variable region between ND2 and ND4 in the mitochondrial genome not only has different gene arrangement order but also has the phenomenon of duplication of ND3 gene which leads to the polymorphism of the mitochondrial genome of C. sinensis The main reason. Based on the above research results and comparison with 6 mitochondrial genomes of C. elegans in GenBank, the basic characteristics of mitochondrial genome are summarized as follows: ①The sequences of mitochondrial genes are different; ② The mitochondrial genes of some nematodes are duplicated Phenomenon, and the number of repetitions is different; ③ there is a big difference in mitochondrial genome size.