论文部分内容阅读
探讨转染IL 3和IL 6基因的骨髓基质细胞系QXMSC1对异基因骨髓移植 (BMT )小鼠免疫功能重建的促进作用。用逆转录病毒载体将小鼠IL 3和IL 6基因转染到骨髓基质细胞系QXMSC1(H 2 d) ,分别建立基质细胞系QXMSC1IL 3、QXMSC1IL 6及QXMSC1IL 3/IL 6 ,供体BALB/c(H 2 d)小鼠骨髓去除T细胞 ,致死量照射 (9 0Gy)受体小鼠C5 7BL/ 6 (H 2 b)后 ,输入供体骨髓细胞 (1× 10 5/只 )的同时输入基质细胞QXMSC1IL 3/IL 6 (5× 10 5/只 )。在骨髓移植后 30d、 6 0d ,检测BMT小鼠脾细胞对LPS、ConA的反应强度、脾细胞中辅助性T淋巴细胞前体频数 (HTLp )、杀伤性T淋巴细胞前体频数 (CTLp )、抗体生成细胞 (PFC )的能力及对迟发型超敏反应 (DTH )的能力来评价BMT后免疫功能的恢复情况。异基因BMT并输入基质细胞系QXMSC1IL 3/IL 6可明显增强BMT后淋巴细胞对LPS、ConA反应强度 ,小鼠对异基因小鼠脾细胞DTH反应增强 ,脾淋巴细胞HTLp、CTLp及PFC数明显增加。基质细胞QXMSC1可作为有效的基因载体明显促进异基因骨髓移植小鼠免疫功能重建。联合转入IL 3和IL 6对BMT后免疫功能的恢复有协同作用
To investigate the role of bone marrow stromal cell line QXMSC1 transfected with IL-3 and IL-6 genes in immune function reconstruction in allogenic bone marrow transplantation (BMT) mice. The murine IL 3 and IL 6 genes were transfected into the bone marrow stromal cell line QXMSC1 (H 2 d) using retroviral vectors to establish the stromal cell lines QXMSC1IL 3, QXMSC1IL 6 and QXMSC1IL 3 / IL 6, respectively, and donor BALB / c (H 2 d) mouse bone marrow to remove T cells, lethal dose (90 Gy) receptor mouse C5 7BL / 6 (H 2 b), and then the donor bone marrow cells (1 × 10 5 cells / mouse) Stromal cells QXMSC1IL3 / IL6 (5 x 105 / only). At 30 days and 60 days after bone marrow transplantation, the response of splenocytes from BMT mice to LPS and ConA, the numbers of helper T lymphocyte precursors (HTLp), CTLp, Antibody-producing cells (PFCs) and their ability to respond to delayed-type hypersensitivity (DTH) to assess the recovery of immune function after BMT. Allogeneic BMT was injected into the stromal cell line QXMSC1IL 3 / IL 6, which significantly enhanced the response of lymphocytes to LPS and ConA after BMT. The DTH response of spleen cells in allogeneic mice was enhanced and the numbers of HTLp, CTLp and PFC in splenic lymphocytes were significantly increased increase. Stromal cells QXMSC1 can be used as a potent gene carrier to promote immune function reconstruction in allogeneic bone marrow transplantation mice. The combined conversion of IL 3 and IL 6 has a synergistic effect on the recovery of immune function after BMT