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目的研究DNA修复基因XRCCl多态性与辐射损伤易感性的关系。方法采用1：1配对病例对照设计,在对唐山市放射人员体检的基础上,以113名出现染色体畸变的射线工作人员为病例组,按性别、年龄(±5岁)、民族、工种配对,选择与病例在同一工作岗位、工龄相等或稍长(≤2年)、累积受照剂量相同或相近且无辐射损伤的放射人员为对照组(113名)。以多聚酶链反应-限制性长度多态性分析技术(PCR-RFLP)检测XRCCl基因,比较不同基因型与辐射损伤易感性的关系。结果病例组XRCCl 26304TT变异基因型频率为18．58%,高于对照组(7．08%),差异有统计学意义(P＜0．05)；携带此种基因型个体发生辐射损伤的风险比携带其他基因型者高3．47倍(OR=3．47,95% CI 1．43～8．44)。XRCCl G27466A和G28152A基因型频率在两组中的分布差异无统计学意义(P＞0．05)。结论XRCCl C26304T基因多态性与辐射致染色体畸变有关联。未发现XRCCl G27466A和G28152A基因多态性与辐射致染色体畸变有关。
Objective To study the relationship between DNA repair gene XRCCl polymorphism and susceptibility to radiation injury. Methods A 1: 1 matched case-control study was designed. On the basis of medical examination of radiographers in Tangshan City, 113 ray workers who had chromosomal aberrations were selected as case groups. By sex, age (± 5 years) Radiographers were selected as the control group (113 patients) with the same job or with the same or slightly longer service life (≤2 years) and the same or similar cumulative exposure dose without radiation injury. The gene XRCCl was detected by PCR-RFLP and the relationship between different genotypes and susceptibility to radiation injury was compared. Results The genotype frequency of XRCCl 26304TT in case group was 18.58%, which was higher than that in control group (7.08%), the difference was statistically significant (P <0.05). The risk of radiation injury in individuals with this genotype 3.47 times higher than those with other genotypes (OR = 3.47, 95% CI 1.43 to 8.44). The frequencies of XRCCl G27466A and G28152A genotypes were not significantly different between the two groups (P> 0.05). Conclusion XRCCl C26304T gene polymorphism is associated with radiation-induced chromosomal aberrations. No XRCCl G27466A and G28152A gene polymorphisms were found to be associated with radiation-induced chromosomal aberrations.