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探讨广东地区幽门螺杆菌（H．pylori）vacA基因亚型的流行情况及不同vacA亚型与H．pylori相关性胃肠疾病的关系。方法：自广东地区不同胃十二指肠疾病患者胃粘膜中分离得到191株H．pylori菌株，抽提各菌株总DNA，用特定引物对各菌株vacA基因的信号序列（s）及中间区等位基因（m）行聚合酶链反应（PCR）检测。结果：广东地区患者H．pylori的vaCA亚型有sla／m2、sla／mlb、slb／m2、sla／mlb-m2、slb／mlb和s2／m26种组合，各亚型所占的比例分别为88．0％（168／191）、7．3％（14／191）、3．1％（6／191）、0．5％（1／191）、0．5％（1／191）和0．5％（1／191）。vacA各亚型在不同H.pylori相关性胃十二指肠疾病中的检出率无显著差异。结论：广东地区H.pylori的vacA基因亚型绝大多数为sla／m2型。不能单纯以vacA亚型作为预测H．pylori感染后临床结局的指标。
To investigate the prevalence of vacA genotype of H.pylori in Guangdong and the relationship between different vacA subtypes and H. pylori isolates. pylori-related gastrointestinal diseases. Methods: 191 H. pylori strains were isolated from gastric mucosa in patients with different gastroduodenal diseases in Guangdong Province. pylori strain. The total DNA of each strain was extracted. The signal sequence (s) and the allele (m) of the vacA gene of each strain were detected by polymerase chain reaction (PCR) with specific primers. Results: Guangdong patients H. The vaCA subtypes of pylori had combinations of sla / m2, sla / mlb, slb / m2, sla / mlb-m2, slb / mlb and s2 / m with 88.0% (1/191), 0.5% (1/191), 0.5% (1/191), 0.5% (1/191), 0.5% 191). The detection rates of vacA subtypes in different H.pylori-associated gastroduodenal diseases were not significantly different. Conclusion: The majority of vacA gene subtypes of H.pylori in Guangdong are sla / m2 type. The vacA subtype can not be used as a predictor. Clinical outcome after pylori infection.