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木薯是一种重要的能源和淀粉作物,具有较强的抗逆性和耐贫瘠等能力。为研究木薯中EIN3基因如何被逆境信号调控,对木薯基因组数据库中的EIN3基因进行了序列分析,表明其具有典型的EIN3-like protein DNA结合结构域;其启动子区具有茉莉酸响应元件等多种响应元件。本研究以木薯品种“华南八号”悬浮培养细胞为材料,利用q RT-PCR检测木薯EIN3基因在乙烯利和茉莉酸甲酯处理后的表达特性。结果显示:EIN3在乙烯处理后,基因表达在8 h内呈现持续上升趋势;而茉莉酸甲酯处理后则整体呈现先上升后下降的趋势,且在2 h基因表达量最大。说明EIN3基因可被乙烯和茉莉酸信号调控,并推测其可受不同激素信号的整合后的综合调控,可参与到植物体内一系列多种生理生化过程。
Cassava is an important energy and starchy crops, with strong resistance to stress and poor resistance. In order to study how the EIN3 gene in cassava is regulated by stress signals, the EIN3 gene in cassava genome database was sequenced and showed that it has a typical EIN3-like protein DNA binding domain; its promoter region has more jasmonic acid responsive elements Response element. In this study, the cassava variety “South China 8” suspension cultured cells as material, using q RT-PCR detection Cassava EIN3 gene expression in ethephon and methyl jasmonate treatment. The results showed that the expression of EIN3 increased continuously within 8 h after ethylene treatment, while the expression of EIN3 increased firstly and then decreased after treated with methyl jasmonate, and reached the highest at 2 h. It shows that EIN3 gene can be regulated by ethylene and jasmonic acid signals and speculated that it can be integrated and controlled by the integration of different hormone signals and can participate in a series of various physiological and biochemical processes in plants.