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以3年生人参幼苗为研究材料,采用盆栽试验设计,研究干旱胁迫时间对人参幼苗叶片相对含水量与质膜损伤状况,以及叶绿素、抗氧化物谷胱甘肽(GSH)含量和抗氧化保护酶(SOD、POD)活性的影响。结果显示:与同期对照(适宜水分处理)相比,干旱胁迫下人参叶片含水量随胁迫时间的延长显著降低;同时,叶绿素与可溶性蛋白含量也随胁迫时间的延长显著下降。丙二醛含量与电解质渗漏较对照组显著增加,并随胁迫时间的延长而升高。抗氧化酶SOD和POD活性高于对照组,随胁迫时间的延长先增加后下降;抗氧化物谷胱甘肽(GSH)含量低于对照组,随胁迫时间的延长呈现降-升-降的变化规律。研究表明,干旱胁迫会对人参幼苗造成一定伤害,但在前期能调动自身抗氧化保护酶类与抗氧化物质提高其抗旱能力,但随着胁迫时间的延长抗氧化能力降低。
Taking the 3-year-old ginseng seedlings as the research materials, the pot experiment design was used to study the effects of drought stress on the relative water content and the plasma membrane damage of the ginseng seedling leaves as well as the contents of chlorophyll, antioxidant glutathione (GSH) and antioxidant enzymes (SOD, POD) activity. The results showed that the leaf water content of leaves under drought stress decreased significantly with the prolongation of stress time compared with that of the same period (suitable treatment with water). Meanwhile, the content of chlorophyll and soluble protein also decreased significantly with prolonging of stress time. Malondialdehyde content and electrolyte leakage than the control group increased significantly, and increased with prolonged stress time. The activity of antioxidant enzymes SOD and POD was higher than that of the control group, and then increased with the prolongation of stress time and then decreased. The content of glutathione (GSH) of antioxidants was lower than that of the control group, and decreased with the prolongation of stress time The law of change. Studies have shown that drought stress will cause some damage to the ginseng seedlings, but in the early stage can mobilize their own antioxidant protective enzymes and antioxidants to improve their ability to resist drought, but with the prolonged stress to reduce the antioxidant capacity.