论文部分内容阅读
目的 探讨CD34和血管内皮生长因子 (VEGF)在人肝癌组织中的表达及微血管密度(MVD)的病理意义。方法 对 30例人肝细胞肝癌 (HCC)及相应癌旁组织 ,10例肝硬化 ,5例轻度慢性肝炎和 4例正常肝组织 ,进行了CD34、VEGF免疫组织化学SP法检测 ,对CD34阳性血管进行MVD计数 ,对VEGF进行半定量计数 ,并结合肝癌的病理特征进行分析。结果 HCC组织中CD34呈广泛 ,窦隙状阳性表达 ,而在正常及慢性肝炎组织中肝窦呈阴性 ,在纤维间隔附近的肝窦壁可见少量散在的CD34弱阳性表达 ,MVD与HCC的肿瘤大小及是否发生门静脉癌栓密切相关 ,瘤块直径 <2cm者的MVD显著低于 >2cm者 ,VEGF阳性率为 83.3 % (2 5 /30 ) ,而在 5例慢性肝炎患者中为阴性表达 ,并与肿瘤发生门静脉癌栓关系密切 (P <0 .0 5 )。瘤旁肝组织亦显示较明显的VEGF阳性 (93 .3% ,2 8/30 )。结论 CD34和VEGF的阳性表达为HCC与非癌性肝组织的鉴别诊断提供了较为可靠的形态学标记。瘤组织可通过自分泌和旁分泌的VEGF促进肿瘤血管形成。
Objective To investigate the expression of CD34 and vascular endothelial growth factor (VEGF) in human hepatocellular carcinoma and the pathological significance of microvessel density (MVD). Methods Immunohistochemical staining for CD34 and VEGF was performed on 30 cases of human hepatocellular carcinoma (HCC) and corresponding paracarcinoma tissues, 10 cases of cirrhosis, 5 cases of mild chronic hepatitis and 4 cases of normal liver tissues. The results were positive for CD34. The blood vessels were counted for MVD, semiquantitatively counted for VEGF, and combined with the pathological features of liver cancer. RESULTS: The expression of CD34 was extensive in HCC tissues, positively expressed in sinusoids, but negative in liver sinusoids in normal and chronic hepatitis tissues. A small amount of scattered CD34 weak positive expression was observed in the sinusoidal septum near the fibrous septum, and the tumor size of MVD and HCC was also found. And whether there is a close relationship between portal vein tumor thrombosis, MVD of tumor block diameter <2cm is significantly lower than> 2cm, VEGF positive rate is 83.3% (2 5/30), and negative expression in 5 patients with chronic hepatitis, and There was a close relationship with the occurrence of portal vein tumor thrombus (P < 0.05). Hepatic tissue around the tumor also showed more obvious VEGF positive (93.3%, 2 8/30). Conclusion The positive expression of CD34 and VEGF provide reliable morphological markers for the differential diagnosis of HCC and noncancerous liver tissue. Tumor tissue can promote tumor angiogenesis through autocrine and paracrine VEGF.