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采用氧化低密度脂蛋白(ox-LDL)诱导的人脐静脉内皮细胞(HUVECs)损伤模型,MTT比色法测定细胞活力,评价茜草炭不同极性部位提取物的抗氧化损伤活性,同时采用超高效液相色谱与串联四级杆飞行时间质谱联用技术(UPLC-Q-TOF-MS)对各极性部位化学成分进行定性研究。结果显示,乙酸乙酯部位与正丁醇部位均可显著提高细胞活力(P<0.01),石油醚部位对细胞活力影响不大,水提取部位对损伤的HUVECs有一定的抑制作用。UPLC-Q-TOF-MS分析结果显示,从茜草炭四个极性部位提取物中共鉴定化合物32种,包括31种醌类及其糖苷类和1个烯萜(茜草哌唑嗪C),石油醚部位、乙酸乙酯部位、正丁醇部位、水提取部位分别鉴定出化合物23、32、26、15种。关联体外细胞实验结果表明,乙酸乙酯部位和正丁醇部位为茜草炭抑制ox-LDL诱导HUVECs损伤的有效部位,为阐明茜草炭的药效物质基础提供科学依据,为茜草炭功效-物质相关性研究奠定基础。
Oxidized low density lipoprotein (ox-LDL) induced human umbilical vein endothelial cells (HUVECs) injury model, MTT colorimetric determination of cell viability, Rubia cordifolia different polarity fractions of the anti-oxidative damage activity, while using super The chemical constituents of each polar site were qualitatively studied by UPLC-Q-TOF-MS with high performance liquid chromatography and tandem quadrupole time-of-flight mass spectrometry. The results showed that both ethyl acetate fraction and n-butanol fraction could significantly increase cell viability (P <0.01). Petroleum ether fraction had little effect on cell viability. Water extract had some inhibitory effects on injured HUVECs. UPLC-Q-TOF-MS analysis showed that 32 species of compounds were identified from the extracts of the four polar fractions of Rubia cordifolia, including 31 quinones and their glycosides and 1 terpene (malathionine C), petroleum Ether, ethyl acetate, n-butanol and water were identified as compounds 23, 32, 26 and 15, respectively. The results showed that ethyl acetate fraction and n-butanol fraction were the effective sites for the inhibition of ox-LDL-induced HUVECs injury by Rubiaceae, providing a scientific basis for elucidating the pharmacodynamic basis of Rubia cordifolia. Research laid the foundation.