论文部分内容阅读
目的:建立树鼩的帕金森病(PD)模型,用行为学和形态学相结合的方法对模型进行验证。方法:应用三种方法制备树鼩PD模型,通过行为学和形态学相结合的方法对模型予以验证。结果:MPTP组树鼩的旱迷宫全天总反应时间、错误反应次数分别比6-OHDA组和罗滕酮组显著增高(P<0.05);MPTP组树鼩的爬杆实验所用时间分别较6-OHDA组和罗滕酮组显著增长(P<0.05)。与树鼩中脑腹侧的对照侧相比,6-OHDA组注射侧和罗滕酮组注射侧中脑腹侧的酪氨酸羟化酶(TH)阳性神经细胞数显著减少(P<0.01);而MPTP组双侧中脑腹侧的TH阳性细胞数均显著减少(对照侧为P<0.01,注射侧为P<0.05)。结论:腹腔注射MPTP制作的树鼩PD模型为较理想的模型,值得在神经科学研究中推广应用。
Objective: To establish a Parkinson’s disease (PD) model of tree shrews and to validate the model by a combination of behavioral and morphological methods. Methods: Three methods were used to prepare the tree 鼩 PD model, and the model was verified by a combination of behavioral and morphological methods. Results: The total reaction time and the number of false reaction in the dry maze in the MPTP group were significantly higher than those in the 6-OHDA group and the rotenone group (P <0.05), respectively. The time spent on climbing the pole in the MPTP group was 6 -OHDA group and rotenone group increased significantly (P <0.05). The number of tyrosine hydroxylase (TH) -positive neurons in the ventral midbrain of the injection side of 6-OHDA group and rotenone group was significantly decreased compared with the control side of the ventral aspect of the tree shrew (P <0.01 ). In the MPTP group, the number of TH-positive cells in ventral midbrain decreased significantly (P <0.01 on the control side and P <0.05 on the injection side). Conclusion: The model of tree 鼩 PD produced by intraperitoneal injection of MPTP is an ideal model and worthy of promotion and application in neuroscience research.