论文部分内容阅读
目的 探讨超声引导下将超声造影剂和人野生型 p5 3基因直接注入瘤体内对大鼠肝癌基因表达的影响。方法 采用免疫抑制法建立大鼠原位肝癌模型。 2 4只实验Wistar大鼠随机分成 4组。第 1组 :超声引导下将基因直接注入瘤体内并不用超声照射 ;第 2组 :注入基因后用超声照射瘤体 ;第 3组 :注入造影剂和p5 3基因后不行超声照射 ;第 4组 :用超声照射注入造影剂和基因的瘤体。 48h后用半定量逆转录 聚合酶链式反应 (RT PCR)测定肝癌细胞内 p5 3mRNA的表达情况。 结果 超声引导下可准确地将基因注入肝癌内 ,4组肝癌细胞内均有 p5 3mRNA的表达 ,第 4组的表达量最高 ,明显高于其他三组 ( P <0 .0 0 1)。第 2组的基因表达量次之 ,高于另外两组 (P <0 .0 5 )。第 1、3组间在外源基因表达上的差异没有显著性意义 (P >0 .0 5 )。结论 超声引导下瘤体内注射超声造影剂和p5 3基因后用超声照射 ,既可有效地控制肝癌基因治疗的靶向性 ,又能提高外源基因的表达量。
Objective To investigate the effects of ultrasound-guided ultrasound-mediated contrast agent injection and human wild-type p53 gene on the gene expression of hepatocellular carcinoma in rats. Methods The rat model of orthotopic liver cancer was established by immunosuppression. 24 Wistar rats were randomly divided into 4 groups. Group 1: Ultrasound-guided injection of gene directly into the tumor without ultrasonic irradiation; Group 2: injection of gene after ultrasound irradiation of tumor; Group 3: injection of contrast agent and p5 3 gene without ultrasonic irradiation; Group 4 : Ultrasound to inject contrast agent and gene tumor. After 48 h, the expression of p5 3 mRNA in hepatocellular carcinoma cells was determined by semi-quantitative reverse transcriptase-polymerase chain reaction (RT PCR). Results The gene could be accurately injected into liver cancer under the guidance of ultrasound. The expression of p5 3 mRNA in the four groups of hepatocellular carcinoma cells was the highest. The expression level of the fourth group was the highest, significantly higher than the other three groups (P <0.01). The second group had the second highest gene expression levels compared with the other two groups (P <0.05). There was no significant difference in exogenous gene expression between the first and third groups (P> 0.05). Conclusion Ultrasound-guided injection of ultrasound contrast agent and p5 3 gene into the tumor after ultrasound-guided ultrasound can not only effectively control the targeting of hepatocellular carcinoma gene therapy but also increase the expression of foreign genes.