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目的了解不同流感病毒型别鸡胚培养的敏感性及分离毒株的生物学特征。方法将流感病例呼吸道标本核酸检测阳性者接种SPF鸡胚,用红血球凝集试验判定分离结果并测定病毒滴度,以血凝抑制试验鉴定毒株型别。结果 802份核酸阳性标本鸡胚分离阳性536份,阳性率66.8%,其中A(H1N1)、A(H3N2)、B和H1N1pdm2009的阳性率分别是52.3%(45/86)、4.3%(3/70)、62.4%(194/311)和87.8%(294/335);首次出现阳性的血凝滴度分别是1∶18.92、1∶15.96、1∶18.83和1∶5.93;合格(血凝滴度≥1∶8)阳性培养物传代数分别为2、2、2和3代。结论鸡胚是流感病毒A(H1N1)和B型的敏感宿主,连续传代有利于提高流感病毒的分离率和血凝滴度。
Objective To understand the sensitivity of different influenza virus types of chicken embryo culture and biological characteristics of isolated strains. Methods SPF chicken embryos were inoculated into the respiratory tract specimens of influenza virus positive for influenza virus. The results of erythrocyte agglutination test and the virus titer were determined. The type of strain was identified by the hemagglutination inhibition test. Results The positive rates of A (H1N1), A (H3N2), B and H1N1pdm2009 in 522 samples were 52.3% (45/86) and 4.3% (3 / 70), 62.4% (194/311) and 87.8% (294/335) respectively. The positive first-time hemagglutination titers were 1:18.92, 1:15.96, 1:18.83 and 1: 5.93, respectively; Degree ≥ 1: 8) The positive culture passages were 2,2,2 and 3 generations respectively. Conclusion Chicken embryo is a sensitive host of influenza A (H1N1) and type B, and continuous passage is conducive to improve the isolation rate of influenza virus and titer of blood clots.