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作者用简易法测定无铅作业接触史的男性工人尿中δ-氨基乙酰丙酸(ALA)的浓度。方法:选择无铅作业接触史的健康男性工人为对象,取其10时～15时任何时间的少量尿,立即用试纸检查尿中糖、蛋白、尿胆素元,同时测尿比重。舍去异常尿。尿样置冰箱(-20℃)中保存。测定时将尿样于37℃下解冻。尿中ALA的测定:取两支具塞比色管A、B,各加尿样1.0毫升,另取1支加ALA标准液(5.0毫克/升),分别向各管加醋酸缓冲液(pH4.6)1.0毫升,然后,向A管及标准管各加乙酰醋酸0.2毫升,用振荡器振荡混合约5秒钟,在沸水浴上加热10分钟生成ALA·吡咯后,冷却。B管不加乙酰醋酸,室温放置,用作空白。再向A、B及标准管各加醋酸乙酯3毫升,用手振摇60次后放置,不易分分层时用3000转/分离心5分钟。取醋酸乙酯层2毫
The authors used the simple method to determine the concentration of δ-aminolevulinic acid (ALA) in the urine of male workers with a history of lead exposure. Methods: Healthy male workers who had no history of lead exposure were enrolled in this study. Take a small amount of urine at any time from 10 o’clock to 15 o’clock, and immediately check the urinary sugar, protein and urobilinogen with test strips and measure urinary specific gravity. Abnormal urine should be discarded. Urine set the refrigerator (-20 ℃) to save. Urine samples were thawed at 37 ° C. Determination of urinary ALA: Take two with a plug colorimetric tube A, B, plus 1.0 ml of urine samples, and the other with ALA standard solution (5.0 mg / L) were added to each tube acetic acid buffer (pH4 .6) 1.0 ml. Then, 0.2 ml of acetoacetic acid was added to each of the A tube and the standard tube. The mixture was shaken by a shaker for about 5 seconds. After heating in a boiling water bath for 10 minutes to produce ALA pyrrole, the mixture was cooled. B tube without acetic acid, placed at room temperature, used as a blank. A, B, and each additional standard ethyl acetate ethyl acetate 3 ml, shake hands after placing 60 times, easy to layer when using 3000 rev / min centrifugation for 5 minutes. Take 2 ml of ethyl acetate layer