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目的:建立测定人血浆中美金刚浓度的高效液相色谱串联质谱法,以用于美金刚人体药动学研究。方法:Ultimate XB-C18柱(150mm×4.6mm,5μm)为色谱柱,甲醇-0.5%甲酸(70∶30)为流动相,流速为0.5mL·min-1;电喷雾电离正离子模式,选择性监测质荷比(M/Z)为180→163(美金刚)和152→135(金刚烷胺,内标);血浆样本经醋酸乙酯提取后进行分析。结果:血浆中美金刚质量浓度在0.2～100μg·L-1范围内线性关系良好,最低定量限达0.2μg·L-1;日内、日间精密度RSD小于7,血浆样本经冷冻和冻融后均稳定。结论:此方法灵敏、专一、快速、简便,适用于盐酸美金刚胶囊人体药动学研究。
OBJECTIVE: To establish a high performance liquid chromatography-tandem mass spectrometry method for the determination of memantine in human plasma for use in human memantine research. Methods: The column of Ultimate XB-C18 (150 mm × 4.6 mm, 5 μm) was used as the column. Methanol-0.5% formic acid (70:30) was used as mobile phase at a flow rate of 0.5 mL · min -1. Electrospray ionization The M / Z values for sexual monitoring were 180 → 163 (memantine) and 152 → 135 (amantadine, internal standard); plasma samples were analyzed after extraction with ethyl acetate. Results: The concentration of memantine in plasma ranged from 0.2 to 100 μg · L-1. The lowest limit of quantification was 0.2 μg · L-1. The intra-day and inter-day RSD was less than 7. The plasma samples were frozen and thawed After all are stable. Conclusion: This method is sensitive, specific, rapid and simple, suitable for human pharmacokinetics of memantine hydrochloride capsules.