以加热灭活的霍乱弧菌(VC)为模型,邻苯二甲酸乙酸纤维素(AquacoatμCPD)为核聚合物,采用喷雾干燥法在60和80℃下制备含或不含海藻酸盐的口服微粒。所得4种微粒外观圆整、表面光滑,平均粒径均约为6μm,载药量8.16%~8.64%,包封率89.77%~94.86%,且抗原性始终保持在85%以上,具有肠溶性质。以大鼠为模型,考察口服给予30或60 mg不同口服微粒(80℃下制得)和VC混悬液后体内免疫反应诱导情况。结果 Using heat-inactivated Vibrio cholerae (VC) as a model and AquacoatμCPD as a core polymer, oral particulates with or without alginate were prepared by spray-drying at 60 and 80 ° C . The results showed that the four kinds of microparticles were round and smooth in appearance with the average particle size of about 6μm, drug loading of 8.16% -8.64%, entrapment efficiency of 89.77% ~ 94.86%, antigenicity more than 85% nature. Using rat as a model, we investigated the induction of immune response in vivo after 30 or 60 mg oral administration of different microspheres (prepared at 80 ° C) and VC suspension. result