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目的 :研究链霉素对豚鼠耳蜗外毛细胞底侧膜 K+ 电流的影响 ,以进一步揭示氨基甙类抗生素耳毒性的离子机制。方法 :全细胞膜片钳制技术。结果 :记录到 Ca2 + 敏感的外向 K+ 电流 [Ik(Ca) ];分别观察了 0 .1,1,10 ,10 0 ,10 0 0 μm ol/ L 链霉素对 Ca2 +敏感的外向 K+电流的影响。在指令电位 + 80 m V时 ,链霉素的抑制率分别为 0 ,(6 .72± 4.42 ) % ,(15 .5 4± 5 .43) % ,(18.0 2± 5 .32 ) % ,(30 .86± 11.2 1) %。结论 :链霉素以浓度依赖方式抑制 Ca2 +敏感的外向 K+电流 ,这可能是其急性耳毒性机制之一。
Objective: To study the effect of streptomycin on the K + current in the basolateral membrane of guinea pig cochlear outer hair cells to further reveal the ionic mechanism of aminoglycoside antibiotics ototoxicity. Methods: Whole cell patch clamp technique. RESULTS: Ca2 + -excited outward K + current [Ik (Ca)] was recorded. The Ca2 + -extracting K + current of 0.1, 1, 10, 10 0 and 10 000 μmol / L streptomycin Impact. The inhibitory rates of streptomycin were (6 .72 ± 4.42)%, (15.5 ± 5.43)%, (18.0 ± 2.32)% at the command potential of +80 mV, (30.86 ± 11.2 1)%. Conclusion: Streptomycin inhibits Ca2 + -dependent outward K + currents in a concentration-dependent manner, which may be one of its acute ototoxicity mechanisms.