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为建立裸鼠胫骨原位骨肉瘤模型,并比较分析MG-63细胞悬液局部注射以及肿瘤组织块移植两种方法的成瘤特性,在注射组体内连续传代筛选MG-63细胞系获得高成瘤率的细胞,将MG-63细胞悬液(25μL,约1×105个)注射于裸鼠胫骨上段髓腔内;组织块移植组将MG-63细胞皮下成瘤瘤组织块接种于裸鼠胫骨上段髓腔内。随后观察两种方法建立的胫骨骨肉瘤原位模型不同的成瘤特性,并采用影像学等手段比较两种方法的成瘤率和成瘤特点。结果表明,术后3周左右可见局部肿瘤形成,4周后通过小动物X线技术可见胫骨中上段溶骨与肿瘤样骨形成,同时在光学显微镜下可见典型的骨肉瘤病理特征。细胞悬液法成瘤率85%,组织块法成瘤率95%,两种方法成瘤率的差异有统计学意义(P<0.05),模型组血清碱性磷酸酶(ALP)明显高于对照组(P<0.01)。两种方法均可形成裸鼠胫骨原位骨肉瘤模型,采用组织块移植法成瘤率较高,瘤体生长速度较快,而且对骨皮质及周围软组织的破坏力较强,转移率高。
In order to establish a model of tibial orthotopic osteosarcoma in nude mice and to compare the tumorigenic characteristics of MG-63 cell suspension local injection and tumor tissue transplantation, MG-63 cell line was serially passaged in the injection group to obtain high For tumor-producing cells, MG-63 cell suspension (25 μL, approximately 1×105 cells) was injected into the upper iliac crest of the tibia; nude mice were inoculated with MG-63 cell subcutaneous tumor tissue in a tissue block transplantation group. Upper humerus in the medullary cavity. Subsequently, the different tumorigenic characteristics of the orthotopic model of tibia osteosarcoma established by the two methods were observed, and the tumorigenesis and tumorigenesis characteristics of the two methods were compared by imaging and other means. The results showed that local tumor formation was seen around 3 weeks after operation. Four weeks later, the middle and upper humerus osteolysis and tumor-like bone formation were seen through small animal X-ray technique. At the same time, typical pathological features of osteosarcoma were seen under light microscope. The tumor formation rate was 85% in the cell suspension method and 95% in the tissue block method. The difference in tumor formation rate between the two methods was statistically significant (P<0.05). The serum alkaline phosphatase (ALP) was significantly higher in the model group than in the model group. Control group (P<0.01). Both methods can form the orthotopic osteosarcoma of the tibia in nude mice. The rate of tumor formation by tissue block grafting is higher, the tumor body grows faster, and the destructive power to the cortical bone and surrounding soft tissues is stronger and the metastasis rate is higher.