目的：观察诱导分化剂苯乙酸对胶质瘤细胞Ｃ６的诱导分化作用，并在分子水平上探讨其作用机理。方法：细胞计数和〔３Ｈ〕－ＴｄＲ掺入法检测细胞增殖，流式细胞术分析细胞周期。结果：苯乙酸对细胞增殖存在剂量（２５ｍｍｏｌ／Ｌ，５０ｍｍｏｌ／Ｌ）依赖性抑制；免疫细胞化学检测ＣＰＭ值降低，肿瘤细胞ＤＮＡ合成减少；细胞周期分析Ｇ０／Ｇ１期所占比例下降，Ｓ期相对升高。结论：苯乙酸可阻抑细胞增殖周期，使细胞周期增殖循环中止于Ｓ期中ＤＮＡ合成准备期Ｓ０期，ＤＮＡ合成减少，抑制细胞增殖。其作用机理为抑制细胞周期中Ｇ１期的ＲＮＡ和蛋白质合成 OBJECTIVE: To observe the induction and differentiation of glioma cell C6 induced by the differentiation agent phenylacetic acid, and to explore its mechanism of action at the molecular level. METHODS: Cell proliferation was measured by cell counting and [3H]-TdR incorporation, and cell cycle analysis was performed by flow cytometry. RESULTS: Phenylacetate inhibited the proliferation of cells in a dose-dependent manner (25mmol/L, 50mmol/L); immunocytochemistry showed that the CPM value was decreased and the DNA synthesis of tumor cells was reduced; the percentage of G0/G1 phase in cell cycle analysis Decline, S phase relative increase. Conclusion: Phenylacetic acid can inhibit the cell proliferation cycle and stop the cell cycle proliferation cycle in the S0 phase of DNA synthesis preparation phase in S phase, reduce DNA synthesis, and inhibit cell proliferation. The mechanism of action is inhibition of RNA and protein synthesis in the G1 phase of the cell cycle