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目的研究我国人类免疫缺陷病毒1型(HIV-1)流行重组株B/CTat基因第一外显子变异特点,探讨这些变化对其功能的影响。方法从确诊的HIV-1感染者全血样本中提取基因组DNA,经套式聚合酶链反应(PCR)扩增和测序。使用GCG和Bioedit软件中的程序进行系统进化树和氨基酸变异分析,同时进行网上三级结构预测。结果总计154份样品中CRF07-BC为120份,CRF08-BC为34份,两种亚型有较广泛的分布,CRF07-BC与标准株的平均基因离散率为(5.748±1.352),CRF08-BC与标准株的平均基因离散率为(1.259±1.931)。结论我国流行重组株CRF07-BC比CRF08-BC有较长的流行时间,CRF07-BC与CRF08-BC基因第一外显子氨基酸相比,主要为半胱氨酸富含区和核心区的变化。在这两个区位点变化可能影响Tat与细胞因子如cyclinT1的结合能力,而成为CRF07-BC在我国流行中获得传播优势的原因。
Objective To study the variation of the first exon of B / CTat gene in the human immunodeficiency virus type 1 (HIV-1) -received recombinant strain in China and to explore the effect of these changes on its function. Methods Genomic DNA was extracted from whole blood samples from confirmed HIV-1 infected patients and amplified and sequenced by nested polymerase chain reaction (PCR). Phylogenetic trees and amino acid variation analysis were performed using the programs in the GCG and Bioedit software while online tertiary structure prediction was performed. Results A total of 154 samples were CRF07-BC 120 and CRF08-BC 34, the two subtypes were widely distributed, the average gene dispersion rate of CRF07-BC and standard strains was (5.748 ± 1.352), CRF08- The average gene dispersion rate between BC and standard strains was (1.259 ± 1.931). Conclusion The prevalence of CRF07-BC in CRF07-BC is higher than that of CRF08-BC in China. The changes of cysteine-rich region and core region of CRF07-BC compared with the first exon of CRF08-BC gene . Changes in these two sites may affect the binding of Tat to cytokines such as cyclinT1, and become the cause of CRF07-BC gaining the spread advantage in our country.