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现在用于治疗糖尿病的胰岛素几乎都是高纯度的,特别强调用人的胰岛素。已有报告用兔血糖法作体内生物测定时,纯的牛胰岛素的降血糖反应比猪或人胰岛素的降血糖反应时间较长,因此建议用纯化的单一来源的标准品来测定相应来源的胰岛素制剂。而且高效液相色谱法(HPLC)和其他理化方法日趋广泛地应用于胰岛素制剂中的含量测定,而这些技术也需要上述新的胰岛素标准品。1982年WHO生物标准化专家委员会提出对高纯度的人、猪、牛胰岛素进行国际协作标定,有16个国家的24个实验室同意参加此项工作,23个参加者提供了实验资料(其中我国参
Insulin now used to treat diabetes are almost always of high purity, with particular emphasis on human insulin. It has been reported that when the in vivo bioassay is performed using the rabbit blood glucose method, pure bovine insulin has a longer hypoglycemic response than pigs or human insulin, it is recommended to use purified single-source standards to determine the corresponding source of insulin preparation. Moreover, high performance liquid chromatography (HPLC) and other physicochemical methods are increasingly used in the determination of insulin preparations, and these techniques also require the above new insulin standard. In 1982, the WHO Committee of Experts on Biological Standardization proposed international collaborative calibration of high-purity human, porcine and bovine insulin. Twenty-four laboratories in 16 countries agreed to participate in the work, and 23 participants provided experimental data (among them,