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目的 研究对比人血白蛋白及四氯化碳 ( CCl4 )两种制备大鼠肝纤维化模型的差别 ,为临床实验研究提供参考。方法 健康 SD大鼠分别用 CCl4 皮下注射法和人血白蛋白免疫法诱导肝纤维化模型 ,观察大鼠生存率、体重、肝功能、血清 PC 、C 、HA水平和肝组织病理学变化情况。结果 白蛋白造模大鼠平均体重较 CCl4 模型大鼠高 ,但动物死亡率高 ,肝纤维化形成率低 ,平均纤维化积分较低 ,与 CCl4 模型比较差异显著 ( P<0 .0 5 )。结论 CCl4 皮下注射法造模简便易行、价格低廉、动物死亡率低、模型成功率高、病变典型 ,可作为肝纤维化实验研究的首选模型。
Objective To compare the difference between two preparations of rat liver fibrosis by human serum albumin and carbon tetrachloride (CCl4), and provide a reference for clinical experimental research. Methods Healthy SD rats were induced by CCl4 subcutaneous injection and human serum albumin immunostaining respectively to observe the changes of survival rate, body weight, liver function, serum PC, C, HA levels and pathological changes in liver tissues. Results Compared with CCl4 model rats, the average body weight of albumin-induced rats was higher than that of CCl4 rats, but the mortality of animals was high, the incidence of hepatic fibrosis was low and the average fibrosis score was lower, which was significantly different from that of CCl4 model (P <0.05) . Conclusion The CCl4 subcutaneous injection method is simple and easy to establish, low in price, low in animal mortality, high in model success rate and typical of disease. It can be used as the first choice model for experimental study of liver fibrosis.