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目的观察长波紫外线(UVA)照射所致人真皮成纤维细胞损伤及黄芪甲苷预处理对细胞的保护作用。方法用20μg/ml黄芪甲苷预处理培养人成纤维细胞,2h后以5、10J/cm2剂量的UVA照射细胞并继续培养24h,使用四甲基偶氮唑蓝还原(MTT)法检测细胞活性,以酶联免疫吸附试验(ELISA)检测IL-6和TNF-α的分泌量,比色法检测细胞上清液中的谷胱甘肽过氧化物酶(GSH-Px)和过氧化氢酶(CAT)含量的变化。结果黄芪甲苷对培养的成纤维细胞无明显毒性;UVA照射24h后,成纤维细胞出现增殖活性下降,IL-6和TNF-α分泌量增加,GSH-Px和CAT活力明显下降,黄芪甲苷处理则可抑制上述改变(均P<0.05)。结论黄芪甲苷预处理可明显抑制UVA照射引起的成纤维细胞活性下降,其机制可能与其有效抑制炎性细胞因子分泌和增强细胞抗氧化能力有关。
Objective To observe the injury of human dermal fibroblasts induced by ultraviolet (UVA) radiation and the protective effect of astragaloside IV on cells. Methods Human fibroblasts were pretreated with 20μg / ml Astragaloside IV and irradiated with UVA at 5,10J / cm2 after 2h. The cells were cultured for 24 hours, and the cell viability was measured by MTT assay The secretion of IL-6 and TNF-α was detected by enzyme-linked immunosorbent assay (ELISA). The contents of glutathione peroxidase (GSH-Px) and catalase (CAT) content changes. Results Astragaloside Ⅳ had no obvious toxicity on cultured fibroblasts. The proliferative activity of fibroblasts decreased, the secretion of IL-6 and TNF-α increased, the activities of GSH-Px and CAT decreased significantly after treated with UVA for 24 h. Astragaloside IV Treatment can inhibit the above changes (all P <0.05). Conclusions Astragaloside IV pretreatment can significantly inhibit the decrease of fibroblast activity induced by UVA irradiation, which may be related to its ability of inhibiting the secretion of inflammatory cytokines and enhancing the anti-oxidative ability of cells.