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目的探讨钙网蛋白(CRT)在大肠癌与癌旁组织中的表达及其意义。方法采用二维色谱与质谱联用技术检测20例大肠癌患者肿瘤组织及癌旁组织CRT的表达情况。结果通过二维色谱与质谱联用技术对大肠癌肿瘤组织及癌旁组织进行分析,对有差异的蛋白质点进行质谱鉴定,发现CRT在癌组织中较癌旁组织中表达升高,并通过免疫印迹进一步验证了其准确性。结论 CRT蛋白的高表达可能与大肠癌的发生、发展有一定的关系。
Objective To investigate the expression and significance of calreticulin (CRT) in colorectal cancer and its adjacent tissues. Methods The expression of CRT in tumor tissues and adjacent normal tissues of 20 patients with colorectal cancer was detected by two-dimensional chromatography and mass spectrometry. Results The colorectal cancer tissues and adjacent tissues were analyzed by two-dimensional chromatography and mass spectrometry, and mass spectrometry identification of differentially expressed protein spots showed that the expression of CRT was higher in cancer tissues than in paracancerous tissues, The blot further validates its accuracy. Conclusion The high expression of CRT protein may have a certain relationship with the occurrence and development of colorectal cancer.