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用火焰光度检测器气相色谱法分离测定生物样品中的硫芥(SM),研究了SM在离体人血、皮肤匀浆中和小猪体内、外的毒物动力学。SM在37℃生理盐水、人和猪的全血、2％皮肤匀浆、猪RBC混悬液和血浆中均以一级过程被消除。SM在生理盐水中的t_(1/2)为4.80min,在其它生物样品中为4-7min,小猪iv SM符合三室开放模型,sc SM呈一级吸收一室模型,pc SM200mg/kg,4h内均未从血中检出SM。SM在小猪体内的毒物动力学特征是:经皮下吸收快,t_(1/2)ka为7.45min;分布快,不到7min室间分布已达到动态平衡,分布广,V_b是机体含水总量的4倍,提示有结合库;消除快,t_(1/2)β为12.0min;SMiv 10mg/kg后2.5min 90％的SM已从循环血中消失。鉴于SM的毒物动力学特征,SM中毒实际情况相抗毒剂硫代硫酸钠又不能透入细胞内,因此,要抢在SM分布、结合之前给药,显然十分困难。
Determination of sulfur mustard (SM) in biological samples by Flame Photometric Detector Gas Chromatography was used to study the toxicokinetics of SM in vitro and in vivo in isolated human blood, skin homogenates and piglets. SM was quenched at 37 ° C in normal saline, whole blood of human and pig, 2% of skin homogenate, porcine RBC suspension, and plasma by a first order procedure. SM in normal saline t_ (1/2) of 4.80min, in other biological samples for 4-7min, iv SM piglets meet the three-compartment open model, sc SM was a primary absorption model, pc SM200mg / kg, 4h were not detected from the blood SM. The toxicokinetics of SM in piglet is characterized by fast subcutaneous absorption, t 1/2 (1/2) ka 7.45min, fast distribution, and less than 7min. The distribution of the compartment has reached a dynamic equilibrium with a wide distribution. V_b is the total water content of the body 4 times the amount, suggesting a binding library; elimination fast, t_ (1/2) β was 12.0min; SMiv 2.5mg after 10mg / kg 90% of the SM has disappeared from the circulating blood. In view of the toxicokinetic characteristics of SM, the actual situation of SM poisoning phase antitoxic agent sodium thiosulfate can not penetrate into the cell, therefore, to grab in the SM distribution, before the combination of administration, obviously very difficult.