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用经过碳离子辐照V79细胞的条件培养基(Irradiatedconditionedmedium,简称ICM)和与受照射细胞共同培养两种方法,研究了未受照射细胞的旁观者效应。结果表明,ICM法可以明显降低未受照射细胞的克隆形成率;受照射细胞与未受照射细胞共同培养一段时间后,细胞克隆形成率比假定没有旁观者效应时的预期值低,细胞微核率和hprt(次黄嘌呤磷酸核糖基转移酶)基因位点突变率与预期值基本相同。推测受照射细胞可能释放出了能对未受照射细胞生长产生毒性的因子,这种因子对未受照射细胞没有明显的致微核和致突变作用。
The bystander effect of unirradiated cells was studied using two methods of Irradiated conditioned media (ICM) irradiated with carbon ions and co-cultured with irradiated cells. The results showed that the ICM method can significantly reduce the non-irradiated cell cloning rate; irradiation and non-irradiated cells co-cultured for a period of time, the cell clone formation rate than the assumption that there is no bystander effect when the expected value of micronuclei Rate and hprt (hypoxanthine phosphoribosyltransferase) gene locus mutation rate with the expected value is basically the same. It is speculated that irradiated cells may release toxic factors that may have toxic effects on the growth of non-irradiated cells. Such factors have no obvious micronuclei and mutagenic effects on non-irradiated cells.