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目的 研究亚低温对缺氧缺血脑损害 (HIBD)新生大鼠的远近期脑保护作用。方法 新生 7天的SD大鼠制作HIBD模型 ,31℃亚低温在HIBD后即刻开始干预 ,干预 6小时 ,采用原位末端标记技术检测脑组织中凋亡细胞数 ,并进行行为观察等 ,研究HIBD新生大鼠近斯脑细胞死亡和远期行为改变 ,以及亚低温对脑的远近期保护作用。结果 新生大鼠HIBD后 ,近期由坏死和凋亡导致神经元死亡 ;远期行为学无统计学意义的改变 (P >0 .1 ) ,但在饥饿诱发下 ,行为能力显著差于假手术组 (P<0 .0 5 ) ;亚低温可使近期凋亡细胞减少 ,8日龄时凋亡细胞数由皮质 47 8± 6 8,海马 37 4± 2 2 4降为皮质 3 0± 2 1 ,海马 3 3± 2 0 (P <0 .0 1 ) ,远期行为学能力改善
Objective To investigate the protective effect of mild hypothermia on neonatal rats with hypoxic-ischemic brain damage (HIBD). Methods 7-day-old SD rats were used to make HIBD model. Mild hypothermia at 31 ℃ began to interfere immediately after HIBD for 6 hours. The number of apoptotic cells in brain tissue was detected by in situ terminal labeling, and the behavior of HIBD was observed. Neonatal rats near brain cell death and long-term behavioral changes, as well as mild and moderate hypothermic brain protective effect. Results Neonatal rats had neuronal death caused by necrosis and apoptosis after neonatal HIBD. There was no significant change in long-term behavior (P> 0. 1), but their behaviors were significantly lower than those in sham operation group (P <0.05). Mild hypothermia could reduce the number of apoptotic cells in the near term. The number of apoptotic cells decreased from 47 8 ± 68 in the cortex to 37 4 ± 2 24 in the hippocampus to 30 ± 21 in the cortex on the 8th day , Hippocampus 3 3 ± 20 (P <0.01), long-term behavioral improvement