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为探索钙对水杨酸(SA)诱导番茄(Solanum lycopersicum)幼苗抗灰霉病的作用,采用‘L402’番茄品种,通过分别喷施8mmol.L-1CaCl2和5mmol.L-1EGTA后再喷施2mmol.L-1SA,3d后接种灰霉病菌孢子的方法,研究了不同处理对番茄幼苗灰霉病病情指数、活性氧积累、主要防御酶活性及其基因表达的影响。结果表明:接种灰霉病菌孢子5d后,SA处理的番茄幼苗病情指数比对照降低37.27%,Ca+SA处理较SA处理进一步降低18%;接种1d和2d后,叶片中产生速率和H2O2含量分别出现应激高峰,且处理间存在差异,与对照相比Ca+SA处理分别提高33.05%和29.31%,EGTA+SA处理分别降低32.62%和46.34%;叶片中抗病相关酶活性和基因表达在接种病菌后也出现应激高峰,其中Ca+SA处理显著提高了PAL、几丁质酶、β-1,3-葡聚糖酶活性,EGTA处理及EGTA+SA处理显著降低了PAL、几丁质酶、β-1,3-葡聚糖酶活性。上述结果表明,Ca2+在SA诱导番茄抗灰霉病中具有正调控作用,而且这种作用与PAL、几丁质酶、β-1,3-葡聚糖酶活性及其基因表达密切相关。
In order to explore the effect of calcium on resistance to Botrytis cinerea in Solanum lycopersicum seedlings, ’L402’ tomato varieties were sprayed with 8mmol.L-1CaCl2 and 5mmol.L-1EGTA respectively 2mmol.L-1SA, 3d after inoculation of Botrytis cinerea spores method to study the tomato seedlings gray mold disease index, reactive oxygen species accumulation, the main defense enzyme activity and gene expression impact. The results showed that after inoculated with Botrytis cinerea sp., The disease index of tomato seedlings decreased by 37.27% compared with the control and the Ca + SA treatment was further reduced by 18% compared with the SA treatment. After 1 and 2 days of inoculation, the production rate and H2O2 content Respectively. The peak of stress appeared in different treatments, and Ca + SA increased by 33.05% and 29.31% compared with the control, while the EGTA + SA decreased by 32.62% and 46.34% respectively. The activity of related enzymes and gene expression The peak of stress also appeared after inoculation with bacteria, in which Ca + SA treatment significantly increased PAL, chitinase, β-1, 3-glucanase activity, EGTA treatment and EGTA + SA significantly reduced the PAL Butylase, β-1,3-glucanase activity. The above results indicate that Ca2 + has a positive regulatory effect on tomato induced resistance to Botrytis cinerea, and this effect is closely related to PAL, chitinase, β-1,3-glucanase activity and gene expression.