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目的观察人乳头状瘤病毒(human papilloma virus,HPV)L1壳蛋白在不同级别鳞状上皮内病变中的表达及意义。方法首次行宫颈液基细胞学检查患者236例,其中低度鳞状上皮内病变178例,高度鳞状上皮内病变50例,鳞状细胞癌8例,采用免疫细胞化学和免疫组织化学法对患者液基薄层细胞学检查制片进行HPV L1壳蛋白检测,并对结果进行统计分析。结果 HPV L1蛋白在表层细胞表达,在基底细胞无表达;HPV LI壳蛋白在低度鳞状上皮内病变中、高度鳞状上皮内病变的宫颈上皮内瘤变Ⅱ与Ⅲ中、以及鳞状细胞癌中的阳性表达率分别为79.8%,41.4%,4.8%,0。结论 HPV L1壳蛋白在不同级别的鳞状上皮内病变中不同表达水平可为临床制订治疗方案、判定预后提供依据。
Objective To observe the expression of human papilloma virus (HPV) L1 capsid protein in different grades of squamous intraepithelial lesion and its significance. Methods The first cervical cytology-based cytology of 236 patients, including 178 cases of low grade squamous intraepithelial lesion, high grade squamous intraepithelial lesion in 50 cases, squamous cell carcinoma in 8 cases, using immunocytochemistry and immunohistochemistry Patients with liquid-based thin-layer cytological examination of HPV L1 capsid protein detection, and the results were statistically analyzed. Results HPV L1 protein was expressed in the surface cells and no in basal cells. HPV L1 capsid protein was found in low grade squamous intraepithelial lesion and in high grade squamous intraepithelial neoplasia Ⅱ and Ⅲ as well as in squamous cell The positive rates of cancer were 79.8%, 41.4%, 4.8%, respectively. Conclusion Different expression levels of HPV L1 capsid protein in different grades of squamous intraepithelial lesion may provide a basis for clinical treatment plan and prognosis.