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采用磺丁基醚-β-环糊精(SBE-β-CD)为手性流动相添加剂,建立了反相高效液相色谱手性流动相添加剂法拆分分离佐匹克隆对映体的方法。在普通C18色谱柱(250 mm×4.6 mm×5.0μm)上,考察了水相pH、磺丁基醚-β-环糊精浓度、磷酸盐缓冲液浓度、甲醇含量、柱温等对佐匹克隆对映体拆分效果的影响。确定最适用的色谱条件:流动相为水相(5 mmol/L NaH2PO4,含磺丁基醚-β-环糊精5 mmo/L,以H3PO4调pH为3):甲醇=78:22(V/V),检测波长305nm,流速为1 mL/min,柱温为30℃,此条件下佐匹克隆对映体的保留时间分别为23.0和25.6 min,分离度为1.81。两对对映体质量浓度在0.04~0.36g/L范围内线性关系良好(r≥0.9990),保留时间的RSD分别为0.73%和0.80%,峰面积的RSD分别为1.2%和1.1%。
A method for the separation and separation of zopiclone enantiomers was developed by using reversed-phase high performance liquid chromatography with chiral mobile phase additive using sulfobutylether-β-cyclodextrin (SBE-β-CD) as a chiral mobile phase additive . The effects of aqueous phase pH, sulfobutylether-β-cyclodextrin concentration, phosphate buffer concentration, methanol concentration and column temperature on zopiclzoic acid were investigated on a common C18 column (250 mm × 4.6 mm × 5.0 μm) Effect of Clone Enantiomers on Resolution. To determine the most suitable chromatographic conditions: the mobile phase was aqueous phase (5 mmol / L NaH2PO4 with sulfobutyl ether-β-cyclodextrin 5 mmo / L, adjusted to pH3 with H3PO4): methanol = 78:22 / V). The detection wavelength was 305 nm, the flow rate was 1 mL / min and the column temperature was 30 ℃. The enantiomeric retention of zopiclone was 23.0 and 25.6 min, respectively, with a resolution of 1.81. The linear relationship between the two enantiomers was good in the range of 0.04-0.36 g / L (r ≥ 0.9990). The RSDs of retention time were 0.73% and 0.80%, respectively. The RSDs of peak areas were 1.2% and 1.1%, respectively.