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Objective: The aim of the study was to explore the effect of STAT5 silenced by siRNA on proliferation, apoptosis and invasion of esophageal carcinoma cell line EC9706. Methods: The siRNA vectors aiming to STAT5 gene were con-structed. STAT5 siRNA was transfected into EC9706 cells by LipofectamineTM 2000. Changes of STAT5, Bcl-2 and Cyclin D1 were analyzed by Western blot and RT-PCR. Effect of STAT5 siRNA on EC9706 cells proliferation was determined by MTT.Effects of STAT5 siRNA on EC9706 cells cycle and apoptosis were detected by the flow cytometry. Boyden chamber was used to evaluate the invasion and metastasis capabilities of EC9706 cells. Results: The double strands oligonucleotide of siRNA aiming to STAT5 was successfully cloned into the pRNAT-U6.1 vector, and the target sequence was coincide with the design. RT-PCR and Western blotting detection demonstrated that the expression levels of STAT5, Bcl-2 and Cyclin D1 genes were obviously decreased in EC9706 cells transfected with STAT5 targeting siRNA expression vectors. STAT5 siRNA could suppress the proliferation of EC9706 cells. The proportions of S and G2/M periods frequency were significantly decreased (P