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本文介绍一种检测抗弓形虫循环抗体IgM的简易方法。实验用弓形虫RH株腹腔接种6～8周龄的雌性小鼠,3天后收集腹腔液中虫株,经洗涤、震荡、离心,使成沉淀物悬液,在500kg/cm~2压力下裂解,再离心洗涤,用氯仿和甲醇(3∶1)混合液脱脂,Folch氏溶剂抽提,再加入三羟基甲烷-盐酸缓冲液和十二烷基磺酸钠、巯基乙醇再行震荡,用Ultragel ACA 44层析,洗脱透析制成样品。而
This article describes a simple method for detecting anti-Toxoplasma circulating IgM. The Toxoplasma gondii RH strain was inoculated intraperitoneally to 6-8 weeks old female mice. After 3 days, the peritoneal fluid was collected, washed, concused and centrifuged, and the precipitate was precipitated and lysed under the pressure of 500 kg / cm ~ 2 , And then washed by centrifugation, degreasing with a mixture of chloroform and methanol (3: 1), extracting with Folch’s solvent, adding trihydroxymethane-hydrochloric acid buffer and sodium dodecyl sulfate, shaking with mercaptoethanol, ACA 44 chromatography, eluting dialysis samples. and