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中药地黄具有滋阴补血,清心滋肾等多种效能,将地黄加工炮制成熟地,其效用更佳。在熟地的有效成分中,糖类具有抗肿瘤病等多种药理作用。但是关于熟地中糖类的提取和测定问题文献未见报道。本文将生地炮制成熟地(其还原糖含量有很大的增加)。然后用85%乙醇溶液在55℃温度条件下分次提取,其中绝大部分可溶性还原糖均被提取出来。与此同时一些水溶性的醇溶性的有色物质及杂质也被提取出来这样就给还原糖比色测定带来困难。在85%的醇溶液中用活性碳脱色效果不好,使比色法测定无法进行,且活性碳对还原糖吸附量很大。采用在低温下蒸去乙醇用水将残留物溶
The traditional Chinese medicine Dihuang has many functions such as nourishing yin and enriching blood, clearing the heart and nourishing the kidneys, etc. It will be processed and matured to make it more effective. Among the effective components of the rehmannia, sugars have various pharmacological effects such as anti-tumor diseases. However, the literature on the extraction and determination of carbohydrates in ripening fields has not been reported. This article will make land to concoct ripe land (its reducing sugar content has a great increase). Then it was extracted several times with 85% ethanol solution at a temperature of 55°C, and most of the soluble reducing sugars were extracted. At the same time, some water-soluble alcohol-soluble colored substances and impurities are also extracted, which makes it difficult to perform colorimetric determination of reducing sugars. In 85% alcohol solution, the effect of decolorization by activated carbon is not good, so that colorimetric determination cannot be performed, and the amount of activated carbon adsorbed on reducing sugar is large. The residue is dissolved by distilling off ethanol at low temperature.