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目的:观察氧化低密度脂蛋白(ox-LDL)对血管平滑肌细胞(VSMC)血管紧张素Ⅱ受体-1(AT1R)表达的影响及凝集素样氧化低密度脂蛋白受体-1(LOX-1)在其中的介导作用。方法:人脐带动脉平滑肌细胞体外原代培养。ox-LDL干预,RT-PCR测定VSMCAT1R mRNA和LOX-1mRNA表达。并观察在LOX-1的抑制剂多聚肌苷酸作用后,VSMCAT1R mRNA表达的变化。结果:①20mg/Lox-LDL作用6h、12h及24h后,VSMCAT1R mRNA表达升高,与空白对照组比较,均P<0.01,作用6h出现峰值。②1、10、100mg/L浓度ox-LDL作用后,VSMCLOX-1mRNA表达均升高,与空白对照组比较,P<0.05～P<0.01;且随着ox-LDL浓度的升高有升高趋势,各浓度之间比较,P<0.01。③多聚肌苷酸抑制LOX-1后,AT1Rm-RNA表达明显下降,与空白对照组、ox-LDL单独作用组比较,均P<0.01。结论:ox-LDL可持续性促进VSMCAT1R表达,LOX-1在此过程中起重要介导作用。
Objective: To investigate the effect of ox-LDL on the expression of angiotensin Ⅱ receptor-1 (AT1R) in vascular smooth muscle cells (VSMCs) and the effect of lectin-like oxidized low density lipoprotein receptor- 1) in which mediation. Methods: Human umbilical artery smooth muscle cells were primarily cultured in vitro. ox-LDL intervention, RT-PCR determination of VSMCAT1R mRNA and LOX-1 mRNA expression. The changes of VSMCAT1R mRNA expression were observed after poly-inosinic acid (LOX-1) inhibitor. Results: ① The mRNA expression of VSMCAT1R increased at 6h, 12h and 24h after treatment with 20mg / Lox-LDL, P <0.01 compared with the blank control group, peaked at 6h. ② The mRNA expression of VSMCLOX-1 in the group of 1, 10 and 100 mg / L ox-LDL increased compared with the control group (P <0.05 ~ P <0.01), and increased with the concentration of ox-LDL , Between the different concentrations, P <0.01. (3) Poly (ADP-riboside) inhibits the expression of AT1Rm-RNA after LOX-1 knockdown, which is significantly lower than that of blank control group and ox-LDL alone group (P <0.01). CONCLUSIONS: Ox-LDL promotes the expression of VSMCAT1R sustainably, and LOX-1 plays an important role in this process.