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目的：探索肿瘤抑制基因ｐ５３、ｐ１６缺失、变异与肿瘤恶性程度的关系。方法：对３１例脑胶质瘤的原发肿瘤标本行ｐ５３、ｐ１６蛋白检测，用免疫组化ＬＳＡＢ法。结果：星形细胞瘤Ⅰ级１７例中，ｐ１６１０例表达３０％以下，７例不表达，ｐ５３４例表达３０％以下，１３例不表达；星形细胞瘤Ⅱ级９例中，ｐ１６１例表达３０％以下，８例不表达，ｐ５３３例表达３０％以下，１例表达３０％～６０％，５例不表达；星形细胞瘤Ⅲ级２例，ｐ１６均不表达，ｐ５３１例表达３０％以下，１例表达３０％～６０％；星形细胞瘤Ⅳ级３例，ｐ１６均不表达，ｐ５３表达均在３０％～６０％。结论：ｐ５３失活主要见于恶性胶质瘤和恶性发展中的胶质瘤，肿瘤的恶性程度越高，ｐ５３蛋白失活越严重。偏良性的胶质瘤ｐ５３呈轻度阳性表达时，提示肿瘤可能处于早期恶性变过程中。ｐ１６缺失主要见于星形细胞瘤Ⅱ、Ⅲ、Ⅳ级等恶性胶质瘤。
Objective: To explore the relationship between tumor suppressor gene p53, p16 deletion and mutation and tumor malignancy. Methods: The primary tumor samples of 31 patients with glioma were detected by p53 and p16 protein, and immunohistochemical LSAB method was used. Results: Among the 17 patients with grade I astrocytoma, p1610 was less than 30%, 7 patients were not expressed, p534 was less than 30%, 13 patients were not expressed. Among the 9 patients with grade Ⅱ astrocytoma, p161 expressed 30 %, No expression in 8 cases, less than 30% in p333 cases, 30% ~ 60% in 1 case and no expression in 5 cases; astrocytoma in grade Ⅲ 2 cases, p16 were not expressed, p531 cases were less than 30% 1 case expressed 30% -60%; astrocytoma Ⅳ grade 3 cases, p16 were not expressed, p53 expression were 30% to 60%. Conclusion: Inactivation of p53 is mainly found in malignant gliomas and malignant gliomas. The higher the degree of malignancy, the more serious the inactivation of p53 protein. The positive expression of p53 in benign glioma was mild positive, suggesting that the tumor may be in the process of early malignant transformation. Mainly found in p16 deletion of astrocytoma Ⅱ, Ⅲ, Ⅳ grade and other malignant gliomas.