论文部分内容阅读
目的 探讨基质金属蛋白酶 -9(MMP -9)的表达和微血管密度与乳腺癌生物学特性的相关性。方法 对原发性乳腺癌组织标本 ,采用标准链霉菌抗生物素蛋白 -过氧化物酶亲合免疫组织法 ,检测肿瘤组织中MMP -9的表达和微血管密度(MVD)。结果 MMP -9的阳性表达率为 42 %;MVD均值为 2 5 .3± 9.1;无腋淋巴结转移组、高分化组、无远处转移组及 5年生存组的MMP -9表达水平分别低于有腋淋巴结转移组、低分化组、远处转移及死亡组 ,有显著性差异 (P <0 .0 5 ) ;MVD值增大提示乳腺癌低分化、发生淋巴结转移和远处转移及预后差 ;MMP -9表达与乳腺癌血管生成有关 ,MMP -9高表达组MVD值大于MMP -9低表达组 (P <0 .0 5 )。结论 MMP -9表达水平及MVD与乳腺癌生长、转移及患者预后相关 ,此结果为临床建立新的乳腺癌预后预测因子和指导临床治疗提供依据。
Objective To investigate the relationship between the expression of matrix metalloproteinase-9 (MMP-9) and the microvessel density and the biological characteristics of breast cancer. Methods The expression of MMP-9 and microvessel density (MVD) in tumor tissue were detected by standard streptavidin-peroxidase immunohistochemistry in primary breast cancer tissues. Results The positive expression rate of MMP-9 was 42% and the average MVD was 25.3 ± 9.1. The expression of MMP-9 in non-axillary lymph node metastasis group, well-differentiated group, distant metastasis-free group and 5-year survival group were lower There was a significant difference (P <0.05) in the group of axillary lymph node metastasis, poorly differentiated group, distant metastasis and death group (P <0.05). The increase of MVD value indicates the poorly differentiated breast cancer, lymph node metastasis and distant metastasis and prognosis The expression of MMP-9 was correlated with the angiogenesis of breast cancer. The MVD in high MMP-9 expression group was higher than that in low MMP-9 expression group (P <0.05). Conclusions The expression of MMP-9 and MVD are correlated with the growth and metastasis of breast cancer and the prognosis of patients. This result provides the basis for establishing a new prognostic factor of breast cancer and guiding the clinical treatment.