论文部分内容阅读
目的 :研究准分子激光原位角膜磨镶术 (laserinsitukeratomileusis ,LASIK)角膜瓣复位后的黏附机制。方法 :实验组 2 4只新西兰兔分为 8组 ,每组 3只 ;对照组 3只。左眼按近视 - 10 .0 0D行LASIK术。分别于术后即刻、2 4h、1周、2周、1月、3月、6月及 12月行裂隙灯显微镜检查 ,并取角膜行光镜观察及免疫组织化学染色 ,检测细胞外基质 (extracellularmatrix ,ECM )成分 ,包括I型胶原、Ⅲ型胶原、Ⅵ型胶原、纤维连接蛋白 (cellularfibronec tion ,cFN)、细胞黏合素 (tenascin ,TN)、Ⅳ型胶原及层黏连蛋白 (laminin ,LN)。结果 :裂隙灯显微镜检查术后角膜瓣贴附紧密 ;光镜观察角膜瓣交界面早期存在缝隙 ,晚期贴附紧密 ;免疫组织化学检测角膜瓣交界面术后早期有cFN的明显表达及Ⅲ型胶原、Ⅵ型胶原的表达 ,无I型胶原、Ⅳ型胶原、LN及TN的表达。结论 :LASIK术后早期 ,角膜瓣与其下的基质层之间的黏附由FN、Ⅵ型胶原及Ⅲ型胶原完成。
Objective: To study the adhesion mechanism of corneal flap after laser in situ keratomileusis (LASIK). Methods: 24 New Zealand rabbits in experimental group were divided into 8 groups, 3 in each group and 3 in control group. Left eye according to myopia - 10 .0 0D LASIK surgery. Slit lamp microscopy was performed immediately after operation, 24 hours, 1 week, 2 weeks, 1 month, 3 months, 6 months and 12 months respectively. Corneal light microscope and immunohistochemical staining were performed to detect the extracellular matrix extracellular matrix (ECM), including collagen type I, collagen type III, collagen type Ⅵ, cFN, tenascin (TN), collagen Ⅳ and laminin (LN) ). Results: The corneal flap was closely attached by slit lamp microscopy. The slit at the early corneal flap was observed by light microscopy and closely adhered at late stage. The expression of cFN in early stage of corneal flap interface was detected by immunohistochemistry and the expression of type Ⅲ collagen , Type Ⅵ collagen expression, no type I collagen, type Ⅳ collagen, LN and TN expression. Conclusion: The adhesion between corneal flaps and the underlying stroma was achieved by FN, type Ⅵ collagen and type Ⅲ collagen in the early stage of LASIK.