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目的:研究血管紧张素Ⅱ1型受体(AT1R)基因A1166C多态性与新疆哈萨克族原发性高血压(EH)的相关关系。方法: 采用多聚酶链式反应法及限制性片段长度多态性技术(PCR-RFLP),对新疆哈萨克族 198 例EH患者(EH组)及130例正常血压者(对照组)的外周血白细胞DNA进行AT1R基因 A1166C多态性检测,观察AA、AC和CC不同基因型以及该位点A、C不同等位基因频率在EH组和对照组中的分布。结果:AA、AC和CC基因型在EH组的分布频率为0.772 8、0.222 1和0.005 1,在对照组为0.761 5、0.238 5 和 0,两组对比差异无统计学意义(P>0.05); A1166 与 C1166 等位基因频率在高血压组中分别为 0.883 8、0. 116 2,对照组中分别为0.880 8和0.119 2,两组相比差异均无统计学意义(P>0.05)。结论:AT1R基因 A1166C分子变异与新疆哈萨克族原发性高血压无相关关系。
Objective: To study the relationship between A1166C polymorphism of angiotensin Ⅱ type 1 receptor (AT1R) gene and essential hypertension (EH) in Kazakstan in Xinjiang Uygur Autonomous Region. Methods: Polymerase chain reaction (PCR) and restriction fragment length polymorphism (PCR-RFLP) were used to detect the leukocyte DNA in 198 EH patients (EH group) and 130 normal persons (control group) The A1166C polymorphism of AT1R gene was detected to observe the distribution of AA, AC and CC genotypes and the frequencies of alleles A and C in EH group and control group. Results: The frequencies of AA, AC and CC genotypes in EH group were 0.772 8, 0.222 1 and 0.005 1, respectively. There was no significant difference between the two groups (P> 0.05) ; Allele frequencies of A1166 and C1166 in hypertension group were 0.883 8,0. 116 2 respectively, and 0.880 8 and 0.119 2 in control group respectively. There was no significant difference between the two groups (P> 0.05). Conclusion: There is no correlation between A1166C mutation in AT1R gene and Kazakh essential hypertension in.