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目的:观察全氟化碳对大鼠心肌缺血再灌注损伤的预防作用。方法:将45只雄性Wistar大鼠随机分为假手术组、缺血再灌注组与全氟化碳组。全氟化碳组在缺血前48h每天腹腔注射全氟化碳(30ml/kg)。结扎大鼠冠状动脉左前降支,使心肌缺血30min,再灌注120min,制成心肌缺血再灌注损伤模型。分别测定血清中乳酸脱氢酶(LDH)、超氧化物岐化酶(SOD)、丙二醛(MDA)、髓过氧化物酶(MPO)水平,并行HE染色观察缺血心肌的病理变化。结果:与假手术组比较,缺血再灌注组LDH、MDA、MPO值均增高,而SOD活性降低(P<0.05);与缺血再灌注组比较,全氟化碳组LDH、MDA、MPO值均降低,而SOD活性增高(P<0.05),且心肌形态学改变更小。结论:全氟化碳对心肌缺血再灌注损伤有预防作用,可能与抗氧化和抗炎症反应有关。
Objective: To observe the preventive effect of perfluorocarbon on myocardial ischemia-reperfusion injury in rats. Methods: Forty-five male Wistar rats were randomly divided into sham operation group, ischemia reperfusion group and perfluorocarbon group. Perfluorocarbons (30ml / kg) were peritoneally injected into the perfluorocarbon group 48 hours before ischemia. Ligation of left anterior descending coronary artery in rats, myocardial ischemia 30min, reperfusion 120min, made of myocardial ischemia-reperfusion injury model. Serum levels of LDH, SOD, MDA and MPO were measured. The pathological changes of ischemic myocardium were observed by HE staining. Results: Compared with the sham-operated group, the LDH, MDA and MPO values in ischemia-reperfusion group increased and the SOD activity decreased (P <0.05). Compared with ischemia-reperfusion group, Value decreased, SOD activity increased (P <0.05), and myocardial morphological changes smaller. Conclusion: Perfluorocarbon can prevent myocardial ischemia-reperfusion injury and may be related to anti-oxidation and anti-inflammation.