1引言研究了甲基紫与核酸分子相互作用的紫外-可见光谱，在pH7左右，核酸包括小牛胸腺DNA，热变性DNA，酵母RNA能够与甲基紫相互作用，甲基紫最大吸收峰在579nm，随着核酸的加入发生明显的减色效应，减色强度随着核酸浓度加大而增强，据此建立测定核酸的新方法，该方法线性范围 1 Introduction The UV-Vis spectrum of methyl violet interaction with nucleic acid molecules has been studied. At pH 7, the nucleic acids including calf thymus DNA, heat-denatured DNA and yeast RNA can interact with methyl violet. The maximum absorption peak of methyl violet 579nm, with the addition of nucleic acid significant reduction effect, subtractive intensity increases with the concentration of nucleic acid increased, thus establishing a new method for the determination of nucleic acid, the linear range of the method