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本文旨在探讨一氧化氮(NO)和烟碱型乙酰胆碱受体(nicotinic acetylcholine receptor,nAChR)在大鼠学习记忆过程中的相互作用。大鼠侧脑室注射NO前体左旋精氨酸(L-Arg)(L-Arg组)或α7nAChR激动剂氯化胆碱(choline chloride,CC组),以及联合注射L-Arg和氯化胆碱(L-Arg+CC组),和先给予α7nAChR拮抗剂甲基牛扁亭(methyllycaconitine,MLA)再联合注射L-Arg和氯化胆碱(MLA+L-Arg+CC组)、先给一氧化氮合酶抑制剂Nω-硝基-L-精氨酸甲酯(L-NAME)再联合注射L-Arg和氯化胆碱(L-NAME+L-Arg+CC组),侧脑室注射生理盐水(NS组)作为对照。用Y型迷宫刺激器检测大鼠的学习和记忆行为能力;用NO试剂盒、免疫组织化学和Western blot分别检测大鼠海马NO含量和α7nAChR及神经元型一氧化氮合酶(neuronal nitric oxide synthase,nNOS)的表达。结果显示,L-Arg+CC组与L-Arg或CC组比较,Y迷宫学习和记忆行为能力均明显增强,同时大鼠海马NO含量、nNOS和α7nAChR表达的光密度值均明显增多;而MLA+L-Arg+CC或L-NAME+L-Arg+CC组与L-Arg+CC组相比较,大鼠学习和记忆行为能力明显减弱,并且海马NO含量及nNOS和α7nAChR的表达均降低。以上结果表明,侧脑室联合应用L-Arg和氯化胆碱可明显提高其单独应用时的大鼠海马NO含量及nNOS和α7nAChR表达,并增强大鼠学习和记忆行为能力;而提前阻断α7nAChR或抑制nNOS后,其联合应用L-Arg和氯化胆碱的效应也得到了抑制。因此推测,NO和nAChR在学习记忆中可能存在着相互协同作用。
This article aims to investigate the interaction of nitric oxide (NO) and nicotinic acetylcholine receptor (nAChR) in learning and memory of rats. Rats were injected intracerebroventricularly with NO-L-Arg (L-Arg group) or α7nAChR agonist choline chloride (CC group), and L-Arg and choline chloride (L-Arg + CC group), and given first a7nAChR antagonist methyllycaconitine (MLA) combined with L-Arg and choline chloride (MLA + L-Arg + CC group) Nitric oxide synthase inhibitor Nω-nitro-L-arginine methyl ester (L-NAME) and then injected L-Arg and choline chloride (L-NAME + L-Arg + CC group) Saline (NS group) served as a control. The learning and memory abilities of rats were detected by Y-type maze stimulator. The levels of NO and α7 nAChR and neuronal nitric oxide synthase (NO) were detected by NO kit, immunohistochemistry and Western blot respectively. , nNOS) expression. The results showed that, compared with L-Arg or CC group, Y-maze learning and memory ability were significantly increased in L-Arg + CC group, while the optical density value of NO content, nNOS and α7nAChR expression in hippocampus were significantly increased in ML group Compared with L-Arg + CC group, L-Arg + CC or L-NAME + L-Arg + CC group significantly decreased learning and memory abilities, and NO content, nNOS and α7nAChR expression in hippocampus decreased. The above results show that the combination of lateral ventricle L-Arg and choline chloride can significantly improve its single use of rat hippocampus NO content and nNOS and α7nAChR expression, and enhance learning and memory behavior; and premature block α7nAChR Or inhibition of nNOS, its combined effect of L-Arg and choline chloride was also suppressed. Therefore, it is speculated that NO and nAChR may play a synergistic role in learning and memory.