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目的 :研究冻融的肿瘤抗原冲击致敏的骨髓来源的树突状细胞 (DC)对结肠癌小鼠是否具有治疗作用。方法 :用小鼠结肠癌细胞株C2 6冻融抗原体外冲击致敏BALB/c小鼠骨髓来源的DC ,观察其体外刺激荷瘤小鼠脾细胞增殖的能力及其诱导的CTL对C2 6肿瘤细胞的杀伤活性 ;体内以 3× 10 5DC /只一次或多次接种于已荷瘤 10d结肠癌的小鼠同侧腹股沟皮下 ,观察抗原冲击的DC对肿瘤生长的抑制作用以及对荷瘤小鼠生存期的影响。结果 :体外抗原冲击致敏的DC能显著刺激荷瘤小鼠脾脏T细胞增殖 ,其诱导的CTL对C2 6肿瘤细胞具有显著的杀伤作用 ,在效靶比为 10∶1,5∶1,2 .5∶1时其杀伤率分别 88 1% ,71.4%和 5 0 .0 % ;抗原冲击致敏的DC体内多次皮下免疫后对肿瘤的生长具有显著的抑制作用 ,能显著延长荷瘤小鼠的生存期。一次性DC体内免疫接种对荷瘤小鼠的治疗作用不明显。结论 :肿瘤细胞冻融抗原体外冲击致敏的DC多次皮下免疫对结肠癌小鼠具有显著的治疗效果
Objective: To study whether bone marrow-derived dendritic cells (DCs) sensitized by freeze-thawing tumor antigens have therapeutic effects on mice with colon cancer. METHODS: Bone marrow-derived DCs of BALB/c mice were sensitized with a freeze-thaw antigen of mouse colon carcinoma cell line C26 in vitro. The ability of in vitro stimulation of the proliferation of tumor-bearing mice spleen cells and the induction of CTL against C26 tumors were observed. The cytotoxic activity of the cells was inoculated intradermally in the inguinal region with 3×10 5 DC/one or more times in mice bearing tumor-bearing 10 d colon cancer, and the inhibitory effect of antigen-pulsed DC on tumor growth and tumor-bearing mice were observed. The impact of survival. RESULTS: DCs sensitized with antigen in vitro could significantly stimulate the proliferation of tumor-bearing mice’s splenic T cells, and the induced CTLs had a significant killing effect on C2 6 tumor cells at a target ratio of 10:1, 5:1, 2 At 5:1, the killing rates were 88 1%, 71.4%, and 50%, respectively. Antigen-pulsed sensitized DCs significantly inhibited the growth of tumors after repeated subcutaneous immunizations in vivo, and could significantly prolong the tumorigenesis. The survival period of rats. One-time DC in vivo immunization had no significant effect on tumor-bearing mice. Conclusion : Multiple subcutaneous immunizations of DCs sensitized by tumor cell freeze-thaw antigen in vitro have significant therapeutic effects on colon cancer mice