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茶树是重要的叶用经济作物,但是茶树每年都要开花结实,消耗大量的养分,导致茶树鲜叶产量减少和品质降低。雌蕊缺失茶树资源是天然的不结实茶树品种。采用Illumina Hi Seq TM 2 500高通量测序技术,对雌蕊缺失茶树花花芽、花蕾、花进行转录组分析。经组装获得237 484条Transcripts,取最长Transcripts作为Unigene,有182 123条,将获得的Unigene与Nr、Nt、Pfam、KOG/COG、Swiss-prot、KEGG、GO等七种数据库进行注释,有22 049条Unigene被注释上26种KOG分类,注释到KEGG的Unigene条数为21 315,涉及的pathway有130条。此外,发掘出与花器官形态建成的ABCDE模型的功能基因31个,这些注释信息的完成为茶树花器官发育基因及性别决定相关基因的发掘提供了重要依据。
Tea trees are important leafy cash crops, but the tea plants are flowering and strong every year, consuming large amounts of nutrients, leading to the reduction of yield and quality of fresh leaves of tea trees. Lack of pistil tea resources are natural and not strong tea varieties. Illumina Hi Seq TM 2 500 high-throughput sequencing was used to analyze the transcriptome of flower buds, flower buds and flowers of the pistil-deficient tea tree. 237484 Transcripts were assembled and the longest Transcripts was taken as Unigene. There were 182 123 Unigene annotated, and the obtained Unigene was annotated with seven databases of Nr, Nt, Pfam, KOG / COG, Swiss-prot, KEGG and GO 22 049 Unigene were annotated on 26 KOG categories, annotating to Kiegel’s Unigene numbering 21 315, and involved in pathway 130. In addition, 31 ABCDE functional genes were found with floral organs morphology. The completion of these annotation information provided important evidences for the developmental genes of genitalia and the genes related to sex determination.