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为明确喜树碱对小菜蛾Plutella xylostella生长发育的抑制作用及其机制,设置0.5、1.0、2.0mg/m L剂量处理其3龄幼虫,采用浸叶饲喂法和高效液相色谱法测定了喜树碱对小菜蛾体内蜕皮激素和保幼激素滴度的影响。结果显示,喜树碱各剂量处理均显著抑制了小菜蛾幼虫的生长发育,抑制率最高达到28.42%;各处理小菜蛾化蛹时间均延迟,异常蛹增多,羽化率降低;其中2.0 mg/m L处理后4 d其化蛹率为6.67%,对照组化蛹率为86.60%。0.5、1.0 mg/m L处理下,小菜蛾体内蜕皮激素滴度显著低于对照组,仅为对照组的0.06倍;而保幼激素滴度增加,分别可达到28.74mg/m L和36.29 mg/m L,是对照组的9倍左右。表明喜树碱可影响小菜蛾体内蜕皮激素和保幼激素的平衡,延迟和影响了小菜蛾的正常发育进程,可实现对小菜蛾种群的控制作用。
In order to clarify the inhibitory effect and mechanism of camptothecin on the growth and development of Plutella xylostella, the third instar larvae were treated with 0.5, 1.0 and 2.0 mg / mL of Lactobacillus bulgaricus by immersion leaf-feeding method and high performance liquid chromatography Effect of camptothecin on ecdysone and juvenile hormone titer in Plutella. The results showed that all treatments of CPT significantly inhibited the growth and development of Plutella xylostella larvae with the highest inhibition rate of 28.42%. The pupation time of Plutella xylostella was delayed and the number of abnormal pupae increased and the emergence rate was decreased. Among them, 2.0 mg / m At 4 days after L treatment, the pupation rate was 6.67% and that in the control group was 86.60%. 0.5 and 1.0 mg / m L treatment, the body ecdysone titers of Plutella xylostella was significantly lower than that of the control group (0.06-fold), while the juvenile hormone titers increased by 28.74 mg / m L and 36.29 mg / m L, which is about 9 times of the control group. Camptothecins could affect the balance of ecdysone and juvenile hormones in the diamondback moth Plutella xylostella, delayed and influenced the normal development of diamondback moth, and could control the diamondback moth population.