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共转导多巴胺合成所必需酶与辅酶基因,使被转导细胞成为自主多巴胺细胞。1 材料和方法 pTH、pGCH、pAADC分别为含酪氨酸羟化酶(TH)、三磷酸鸟苷环水解酶I(GCH)、芳香族氨基酸脱羧酶(AADC)基因的腺伴随病毒(AAV)载体质粒。以磷酸钙共沉积
Co-transduction of dopamine necessary for the synthesis of enzymes and coenzyme genes, the transduced cells become autonomous dopamine cells. 1 Materials and methods pTH, pGCH and pAADC were adeno-associated virus (AAV) containing tyrosine hydroxylase (TH), guanosine triphosphate cyclohydrolase I (GCH) and aromatic amino acid decarboxylase (AADC) Vector plasmid. Calcium phosphate co-deposition