研究肿瘤细胞生物学特性的重要手段之一是建立肿瘤细胞株。迄今,虽然已建立了许多肿瘤细胞株,但是具有独特生物学以及生物化学特性的细胞株仍不断有人报导。早期建立细胞株的主要困难有三点:一是微生物污染。二是培养细胞对复杂营养索的要求。三是间质细胞的过度生长,目前两个问题已基本得到克服,最困难的是如何除去过度生长的间质细胞(纤维细胞)。因为如不 One of the important means to study the biological characteristics of tumor cells is to establish tumor cell lines. To date, although many tumor cell lines have been established, cell lines with unique biological and biochemical characteristics are still being reported. There are three main difficulties in the early establishment of cell lines: First, microbial contamination. The second is to cultivate cell requirements for complex nutrition lines. The third is the excessive growth of mesenchymal cells. At present, the two problems have basically been overcome. The most difficult problem is how to remove the excessively growing mesenchymal cells (fibroblasts). Because if not