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目的探讨母鼠及断乳后高脂饮食对子代雄鼠(下称“子鼠”)糖脂代谢和肠道菌群的影响。方法 C57BL/6J小鼠在孕期、哺乳期及子鼠断乳后分别持续予高脂饲料或正常饲料,至32周龄观察子鼠糖脂代谢指标及肠道菌群的变化。结果子鼠断乳时,高脂饮食(HF)组母鼠较正常饮食(Con)组母鼠体重更重(P<0.05),而FPG比较,差异无统计学意义。32周龄时,HF组子鼠较Con组子鼠体重更重(P<0.05),且出现糖耐量异常和IR。HF组子鼠TG和TC水平高,并出现肝脏脂肪变性。肠道菌群分析结果表明,HF组子鼠肠道菌群生物多样性低于Con组子鼠。在门的水平上,HF组子鼠软壁菌门和拟杆菌门比例下降,而变形菌门升高[(1.13±0.09)%vs(6.47±1.85)%](P<0.05)。在属的水平上,粪球菌属、瘤胃球菌属、普氏菌属、乳杆菌属和不可培养属比例均下降,而拟杆菌属比例升高[(7.14±1.58)%vs(24.30±5.28)%](P<0.05)。结论母鼠及断乳后高脂饮食能导致子鼠糖脂代谢异常和肠道菌群失调。
Objective To investigate the effects of high fat diet on the metabolism of glycolipids and intestinal microflora in offspring of offspring (“squirrel”). Methods C57BL / 6J mice were fed with high fat diet or normal diet respectively during pregnancy, lactation and weaning, and the indexes of glucose and lipid metabolism and intestinal flora were observed at 32 weeks old. Results At the time of weaning in the offspring, the body weight of the female rats in the HF group was heavier than that in the normal group (P <0.05). However, there was no significant difference in FPG between the two groups. At 32 weeks of age, HF rats were heavier (P <0.05) than Con rats, and had impaired glucose tolerance and IR. HF group of rats TG and TC levels, and fatty degeneration of the liver. Intestinal flora analysis showed that the intestinal flora of HF group was lower than that of Con group. On the level of the door, the proportion of the soft-shelled and the bacteroides in the HF group decreased, while that of the proteobacteria in the HF group increased [(1.13 ± 0.09)% vs (6.47 ± 1.85)%] (P <0.05). On the genus level, the proportion of Bacteroides, Rumenococcus, Prevotella, Lactobacillus and non-culturable gene decreased while the proportion of Bacteroides increased [(7.14 ± 1.58)% vs (24.30 ± 5.28) %] (P <0.05). Conclusion Dairy rats and post-weaning high-fat diet can lead to dyslipidemia and intestinal flora dysfunction in the offspring.